4-chlorobenzoyl chloride

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Ames test without directly given guideline. Test including two bacteria in several strains (S. typhimurium TA 100, TA 1535, TA 1537, TA 1538, TA 98, E. coli WP2uvra), with and without metabolic activation. Dose finding tests via cytotoxicity

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

p-Chlorbenzoylchlorid

IUPAC Name:

p-Chlorbenzoylchlorid

Constituent 2

Reference substance name:

4-chlorobenzoyl chloride

EC Number:

204-515-3

EC Name:

4-chlorobenzoyl chloride

Cas Number:

122-01-0

Molecular formula:

C7H4Cl2O

IUPAC Name:

4-chlorobenzoyl chloride

Constituent 3

Reference substance name:

Benzoylchloride, 4-chloro

IUPAC Name:

Benzoylchloride, 4-chloro

Details on test material:

Purity 99%

Method

Target gene:

In the Salmonella typhimurium strains (TA 1535, TA 100, TA 1537, TA 1538, TA 98) the amino acid histidine locus is the target gene.
In the Escherichia coli strain (WP2 uvrA) the amino acid tryptophan locus is the target gene.

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium, other: TA 1535, TA 100, TA 1537, TA 1538, TA 98

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A

Metabolic activation:

with and without

Metabolic activation system:

Rat liver S-9 mix from Arochlor 1254 induced rats

Test concentrations with justification for top dose:

0, 4, 20, 100, 500, 2500, 10000 µg/plate

Vehicle / solvent:

DMSO

Controls

Untreated negative controls:

yes

Positive controls:

yes

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Test results 2

Species / strain:

S. typhimurium TA 1537

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Test results 3

Species / strain:

S. typhimurium TA 1538

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Test results 4

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Test results 5

Species / strain:

S. typhimurium TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Test results 6

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Additional information on results:

The test compound proved to be toxic to the bacterial strains at 500µg/plate.

Applicant's summary and conclusion

Conclusions:

p-Chlorobenzoyl chloride is not mutagenic in these bacterial test systems either with or without exogenous metabolic activation at the dose levels investigated.