Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-537-0 | CAS number: 107-96-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-02-29 to 2008-04-01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Swiss Federal Office of Public Health, Consumer protection directorate, Notification Authority for Chemicals, 3003 Bern, Switzerland
- Analytical monitoring:
- yes
- Details on sampling:
- For measurement of the actual concentrations of the test item, duplicate samples were taken from the test media of all test concentrations at the start of the test (without algae) and at the end of the test (containing algae). At the same sampling times, duplicate samples were also taken from the control.
For sampling at the end of the test, the test medium of the treatment replicates was pooled. Immediately after sampling, all samples were buffered to pH 5 (for details see analytical phase report) in order to stabilize the latter during the storage period. Thereafter, all samples were stored deep-frozen (at about -20°C). In pre-experiments for investigation of the storage stability of the samples (non-GLP), the test item proved to be stable under these storage conditions. - Vehicle:
- no
- Details on test solutions:
- The test medium of the highest nominal concentration of 100 mg/L was prepared by dissolving 100.7 mg of the test item completely in 1000 mL of test water using intense stirring for 15 minutes at room temperature. The test medium of the highest test concentration was diluted with test water to prepare the test media of the lower test concentrations. The test media were prepared just before the start of the test.
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata (Formerly Selenastrum capricornutum)
- Strain: 61.81 SAG
- Source (laboratory, culture collection): Collection of Algal Cultures, University of Goettingen, Germany
- Age of inoculum (at test initiation): inoculum culture was set up three days before the start of the test
- Method of cultivation: cultivation in RCC's laboratories under standardised conditions according to the test guidelines
ACCLIMATION
- Culturing media and conditions (same as test or not): same as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- calculated: 0.24 mmol/L (= 24 mg/L as CaCO3)
- Test temperature:
- 22°C; for measured values see below ("remarks on results including tables and figures");
Water temperature of test media recorded daily. - pH:
- for measured values see below ("remarks on results including tables and figures");
pH was measured in each treatment at the start and end of the test. - Dissolved oxygen:
- not determined (not recommended by the algae guidelines)
- Nominal and measured concentrations:
- nominal (mg/L): 100, 46, 22, 10, 4.6, 2.2 , 0 (control)
initially measured (mg/L): 78, 30, 19, 8.0, 4,1, n.d., 0 (control) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, fill volume: glass, 50 mL, 15 mL
- Aeration: no
- Initial cells density: 10000 algal cells/mL
- Control end cells density: increase by a factor of 124 (over 72 h)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): no vehicle control
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Photoperiod: 24 h/day
- Light intensity: approx. 6400 Lux (range: 5800-7000 Lux) (continuous illumination with fluorescent tubes (Philips TLD 36W/840))
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorimeter
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.145 - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (tested separately twice a year)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 32.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: nomonal, recalculated based on mean recovery
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 21.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: recalculated based on mean recovery
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 17 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence intervals: 6.0-21 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 19 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence intervals: 9.8-23 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 26 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence intervals: 21-34 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.1 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 8 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- corresponding 72 hour EyC values (yield; also based on initial measured test item concentrations), in mg/L (values in brackets are the 95% confidence intervals):
EC10: 6.8 (0.00-12)
EC20: 9.0 (0.01-14)
EC50: 16 (2.5-30)
NOEC: 4.1
LOEC: 8.0 - Results with reference substance (positive control):
- The result of the latest positive control test performed in 2007 (RCC Study No. B67342) showed the sensitivity of the test system was within the historical range of the laboratory: 2007 test: 72-hr EC50 for growth rate = 1.20 mg/L Range of the 72-hr EC50 for growth rate from 2000 to 2007: 0.71 - 1.74 mg/L.
- Reported statistics and error estimates:
- The 72 h EC10, EC20 and EC50 values for the inhibition of average growth rate and yield and their 95% confidence intervals were calculated by Probit Analysis. For the determination of the LOEC and NOEC, average growth rate and yield at the test concentrations were compared to the control values by Dunnett's tests.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The effect levels based on nominal concentrations are: ErC10 = 21.3 mg/L; ErC50 = 32.6 mg/L; NOEC = 4.6 mg/L
- Executive summary:
The influence of the test item 3-Mercaptopropion ic acid on the growth of the freshwater green algal species Pseudokirchneriella subcapitata (formerly Selenastrum capricornutum) was investigated in a 72-hour static test according to the OECD Guideline 201 (2006) and the EU Commission Directive 92/69/EEC, C.3 (1992).
The nominal concentrations of the test item of 2.2, 4.6, 10, 22, 46 and 100 mg/L were tested in parallel with a control.
The measured concentrations of the test item in the freshly prep ared test media of the nominal concentrations of 2.2 to 100 mg/L were betw een 65 and 90% of the nominal values. After 72 hours test duration, the concentrations of the test item were determined to be below the LOQ (1.27 mg/L) at the nominal test concentrations of 4.6 to 46 mg/L. At the highest nominal test concentration of 100 mg/L, 45% of the nominal value was found at the end the test period.The test item had a significant inhibitory effect on the growth of the algae (growth rate and yield) after the test period of 72 hours at the concentr ation of 8.0 mg/L (initially measured) and at all higher test concentrations. The microscopic examination of the algal cells at the end of the test showed no difference between the algae growing at the nominal test concentration of 100 mg/L and the algal cells in the control.
The results can be summarized as follows (on the basis of the initially measured concentrations of the test item): ErC10 = 17 (6.0-21) mg/L, EcR50 =26 (31-34) mg/L, NOErC = 4.1 mg/L.
Due to instability of the substance in aqueous media and since the transformation products are less toxic than the parent substances (no free –SH groups) regulatory endpoints calculated on the basis of nominal concentrations represent a realistic worst case approach.
The effect levels based on nominal concentrations are: ErC10 = 21.3 mg/L; ErC50 = 32.6 mg/L; NOEC = 4.6 mg/L
Reference
The test item could be shown to be instable at pH > 6.0 (pre-experiments, non-GLP). As the pH of all test concentrations (except 100 mg/L) was > 6.0 and additionally the algal growth causes an increasing pH, the degradation/modification (probably oxidation [6]) of the test item could not be avoided. However, pretests with the modified test item at pH 8 have shown also 100% inhibition of the algal growth. Thus, it can be assumed that the modified test item shows a similar toxicity as the parent compound at low pH. Therefore, the following biological results are based on the initial measured concentrations of the test item.
The measured concentrations of the test item in the freshly prepared test media of the nominal concentrations of 2.2 to 100 mg/L were between 65 and 90% of the nominal values, as shown below:
Nominal test conc.(mg/L) |
Initial measured conc. test item |
|
(mg/L) |
(% of nominal) |
|
2.2 |
n.d. |
- |
4.6 |
4.1 |
90 |
10 |
8.0 |
80 |
22 |
19 |
88 |
46 |
30 |
65 |
100 |
78 |
78 |
After 72 hours test duration, the concentrations of the test item were determined to be below the LOQ (1.27 mg/L) at the nominal test concentrations of 4.6 to 46 mg/L. At the highest nominal test concentration of 100 mg/L, 45% of the nominal value was found at the end the test period.
The test item had a significant inhibitory effect on the growth of the algae (growth rate and yield) after the test period of 72 hours at the concentration of 8.0 mg/L (initially measured) and at all higher test concentrations (results of Dunnett's tests, one-sided, = 0.05). Thus, this concentration was determined to be the 72-hour LOEC.
The 72-hour NOEC was determined to be 4.1 mg/L (initially measured), since up to and including this test concentration the growth rate and yield of the algae after 72 hours were not significantly lower than in the control.
The following biological results are based on the initial measured concentrations (mg/L) of the test item:
Parameter (0-72 hr) |
Growth Rate |
Yield |
EC10 (95%CI) |
17 (6.0-21) |
6.8 (0.00-12) |
EC20 (95%CI) |
19 (9.8-23) |
9.0 (0.01-14) |
EC50 (95%CI) |
26 (21-34) |
16 (2.5-30) |
NOEC |
4.1 |
4.1 |
LOEC |
8.0 |
8.0 |
Mean measured light intensity: approximately 6400 Lux (range of 5800 - 7000 Lux). At the start of the test, the pH measured in the treatments was between 5.4 and 8.4. At the end of the test, pH values of 6.2 to 9.3 were measured. The increase of the pH during the test was caused by the uptake of CO2 by the algae due to their growth. The water temperature during the test was 22 °C.
The microscopic examination of the algal cells at the end of the test showed no difference between the algae growing at the nominal test concentration of 100 mg/L and the algal cells in the control. The shape and size of the algal cells was obviously not affected by the test item up to at least this concentration.
No remarkable observations were made concerning the appearance of the test media. All test media were clear solutions throughout the test period.
All validity criteria for OECD TG 201 were met.
Description of key information
EC50 (72 h, growth rate) = 26 mg/L (Pseudokirchneriella subcapitata);
NOEC (72 h, growth rate) = 4.1 mg/L (Pseudokirchneriella subcapitata)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 26 mg/L
- EC10 or NOEC for freshwater algae:
- 4.1 mg/L
Additional information
The influence of the test item 3-Mercaptopropion ic acid on the growth of the freshwater green algal species Pseudokirchneriella subcapitata (formerly Selenastrum capricornutum) was investigated in a 72-hour static test according to the OECD Guideline 201 (2006) and the EU Commission Directive 92/69/EEC, C.3 (1992).
The nominal concentrations of the test item of 2.2, 4.6, 10, 22, 46 and 100 mg/L were tested in parallel with a control.
The measured concentrations of the test item in the freshly prep ared test media of the nominal concentrations of 2.2 to 100 mg/L were betw een 65 and 90% of the nominal values. After 72 hours test duration, the concentrations of the test item were determined to be below the LOQ (1.27 mg/L) at the nominal test concentrations of 4.6 to 46 mg/L. At the highest nominal test concentration of 100 mg/L, 45% of the nominal value was found at the end the test period.
The test item had a significant inhibitory effect on the growth of the algae (growth rate and yield) after the test period of 72 hours at the concentr ation of 8.0 mg/L (initially measured) and at all higher test concentrations. The microscopic examination of the algal cells at the end of the test showed no difference between the algae growing at the nominal test concentration of 100 mg/L and the algal cells in the control.
The results can be summarized as follows (on the basis of the initially measured concentrations of the test item): ErC10 = 17 (6.0-21) mg/L, EcR50 =26 (31-34) mg/L, NOErC = 4.1 mg/L.
Due to instability of the substance in aqueous media and since the transformation products are less toxic than the parent substances (no free –SH groups) regulatory endpoints calculated on the basis of nominal concentrations represent a realistic worst case approach.
The effect levels based on nominal concentrations are: ErC10 = 21.3 mg/L; ErC50 = 32.6 mg/L; NOEC = 4.6 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
