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EC number: 800-940-9
CAS number: 35836-72-7
on Growth Rate
An algal toxicity study was performed in
accordance with OECD Guideline No 201 and GLP. Following a preliminary
range-finding test, Pseudokirchneriella subcapitata algae were exposed
to nopyl acetate at nominal concentrations of 1.0, 1.8, 3.3, 6.0, 11 and
20 mg/L (three replicate flasks per test concentration and six
replicates for control) for 72 h, under constant illumination and
shaking at a temperature of 22.7-23.2 °C. Measurements of biomass,
growth inhibition and yield were done by determination of cell densities
at 24, 48 and 72 h using a counting chamber.
After 72 h, cell concentration in the
control culture increased by 101 folds; mean CV for section by section
specific growth rate was 28 and CV for average specific growth rate was
3.4 %. Therefore the control met the validity criteria for the control
The concentrations of the test item (with
and without algae) were measured at the start of the study and at the
72-h incubation period. The concentrations were not satisfactorily
maintained within ± 20% of the initial concentrations, thus the results
were based on the geometric mean of measured exposure concentrations in
the abiotic test.
Therefore, based on measured concentrations
of nopyl acetate, the ErC50(72 h) and EyC50 (72 h)
for green algae (Pseudokirchneriella subcapitata) were determined to be
7.11mg/L and 3.03 mg/L respectively.
Based on the results of this study, nopyl
acetate is classified:
- dangerous for the environment according to
Directive 67/548/EEC "R51/53: toxic to aquatic organisms, may cause
long-term adverse effects in the aquatic environment"
- and hazardous to the aquatic environment
under Regulation (EC) No 1272/2008 (CLP) - Category Chronic 2.
An algal toxicity study was performed in accordance with OECD Guideline No 201.The 72h-ErC50 of nopyl acetate for freshwater algae is 7.11 mg/L.The 72h-ErC10 of nopyl acetate for freshwater algae is 2.59 mg/L.Based on the results of this study, nopyl acetate is classified: - dangerous for the environment according to Directive 67/548/EEC "R51/53: toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment" - and hazardous to the aquatic environment under Regulation (EC) No 1272/2008 (CLP) - Category Chronic 2.
Key study conducted according to OECD 201
and EU C3 Guidelines under GLP without deviation.
Algae cultures (Pseudokirchneriella
subcapitata) were exposed to nopyl acetate at nominal concentrations of
1.0, 1.8, 3.3, 6.0, 11 and 20 mg/L over a test period of 72 hours
(static test). Concentrations were measured at the start of the study
and daily until the end of the test. The concentrations of the test item
were not satisfactorily maintained within ± 20% of the initial
concentrations throughout the test, thus the EC50 were based
on the geometric mean of the measured concentrations in abiotic
replicates. The 72h-ErC50 and the 72h-ErC10
obtained in this study can be used as key values for chemical safety
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