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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

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Hydrolysis as a Function of pH.

No determination was possible according to Method C7 Degradation - Abiotic Degradation, Hydrolysis as a Function of pH of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 111 of the OECD Guidelines for Testing of Chemicals, 13 April 2004, due to the following reasons:

  • The test item is a complex reaction mixture. The test is not appropriate for complex reaction mixtures.
  • The test item was estimate to be essentially insoluble in water. The method guidelines state that extremely water-insoluble substance may not be suitable for hydrolysis testing.

Biodegradation

Two biodegradation studies were carried out on the test material:

Clarke (2012) - Enhanced Biodegradation Screening Test

A study was performed to assess the biodegradability of the test item in an aerobic aqueous medium. The test method was designed to follow OECD Guidelines for Testing of Chemicals (1992) No 301B, "Ready Biodegradability; CO2Evolution Test" referenced as Method C.4-C of Commission Regulation (EC) No. 440/2008 and US EPA Fate, Transport, and Transformation Test Guidelines OPPTS 835.3110 (Paragraph (m)). However following the recommendations of the ECHA REACH Guidance Document “Guidance on information requirements and chemical safety assessment: Chapter R.7b: Endpoint specific guidance” the following modifications to a standard OECD 301B test were made:

  • The test duration may be extended up to 60 days dependent on the level of biodegradation observed.
  • Testing in larger vessels: The test volume employed was increased from 3 litres to 4 litres.
  • Increasing the biomass: The concentration of inoculum used was increased from 30 mg suspended solids (ss)/l to 50 mg ss/l.


The test item attained 31% degradation after 28 days and 77% degradation after 60 days.

Brendgen (1997) - Biodegradation Test Results for the Test Substance PR-471.

Biodegradation testing has been performed on the test substance PR-471 based on OECD guideline 306 for testing chemicals: " Biodegradability in seawater" - Closed Bottle Test. The test substance PR-471 was degraded by 28% after 28 days based on COD calculations and measurements to account for O2 consumption by nitrification.

The latter study (Clarke 2012) is considered to be the key study as this has been carried out according to the most recent Guidelines.


Adsorption Coefficient. No determination was possible according to Method C19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001, due to the following reasons:

  • An assessment of the emulsification properties of the test item was carried out with the test item prepared in water saturated n-octanol (at 1.04 g/L) and shaken with an equal amount of n-octanol saturated water. Observations after shaking indicated that the two immiscible phases emulsified easily; additionally, after 90 minutes standing, the upper phase was a very densely white opaque emulsion, the lower layer being a translucent white liquid. It was therefore considered that the test item was surface-active. Surface-active items are not suitable for determination by the HPLC method.
  • The dissociation constants of the imidazoline and amine functional groups were estimated to be in the range approximately 10.9 to 5.7. Therefore, the test item would maintain at least one cationic charge over the entire environmentally relevant pH range of 5.5 to 7.5 applicable for the method. However, cationic species have been demonstrated to interact with the HPLC column stationary phase by mechanisms other than partitioning, thus invalidating the estimation of the adsorption coefficient of the test item by comparison to the capacity factors of reference substances of known adsorption coefficient values.

Therefore, the adsorption coefficient of each set of components was estimated using a computer-based atom fragment contribution method, KOCWIN, version 2.00 (February 2009), (C) 2000 -2008 US Environmental Protection Agency.

Components

Estimated log10Koc

MCI method

Kow method

A

~5.1

~4.6

B

~10.0

~9.7

C

~4.3 to ~5.3

~3.3 to ~4.4

D

~8.1 to ~9.2

~7.5 to ~8.6

E

~5.9

~5.5