Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-597-4 | CAS number: 1215841-86-3
The test substance trans-N1,N1,N4,N4-Tetrakis(2-hydroxyethyl)1,4-cyclohexanedicarboxamide was examined for its potential to induce gene mutations in the plate incorporation test and the pre-incubation test using the Salmonella typhimurium strains TA 1535, TA 1537, TA 98, TA 100 and TA 102, with and without the addition of a metabolising system (Arochlor 1254 induced rat liver S9 mix)
trans-N1,N1,N4,N4-Tetrakis(2-hydroxyethyl)1,4-cyclohexanedicarboxamide was examined in two preliminary cytotoxicity tests (plate incorporation test without and with metabolic activation) in test strain TA 100. Ten concentrations ranging from 0.316 to 5000 µg/plate were tested. No signs of cytotoxicity were noted up to the top concentration of 5000 µg/plate.
Hence, 5000 µg of the test item/plate was chosen as top concentration for the main study in the plate incorporation test and in the preincubation test, respectively.
Six concentrations ranging from 31.6 to 5000 µg trans-N1,N1,N4,N4-Tetrakis(2-hydroxyethyl)1,4-cyclohexanedicarboxamide/plate were employed in two independent experiments each carried out without and with metabolic activation.
No signs of cytotoxicity were noted in the plate incorporation test or in the preincubation test, each carried out without and with metabolic activation, up to the top concentration of 5000 µg/plate in all test strains.
No mutagenic effect (no increase in revertant colony numbers as compared with control counts) was observed for the test item, tested up to aconcentrationof 5000 µg/plate,in any of the 5 test strains in two independent experiments without and with metabolic activation, respectively (plate incorporation and preincubation test).
In conclusion, under the present test conditions trans-N1,N1,N4,N4-Tetrakis(2-hydroxyethyl)1,4-cyclohexanedicarboxamide tested up to a concentration of 5000 µg/plate, caused no mutagenic effect in theSalmonella typhimuriumstrains TA 98, TA 100, TA 102, TA 1535 and TA 1537 neither in the plate incorporation test nor in the preincubation test each carried out without and with metabolic activation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again