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EC number: 213-254-4 | CAS number: 932-64-9
There was no change in the test substance, NTO/MTT solution or the water control/MTT solution after three hours incubation in the dark at 37 +/- 2oC in a humidified atmosphere of 5% CO2 in air. The test substance had not interacted with the MTT.
Negative control: the mean absorbance of the triplicate negative control values was 0.916 which was between the minimum and maximum values of 0.6 and 1.5. The standard deviation (SD) of the % viability was 7.6 which was below the maximum value of 18.
Positive control: The percentage mean viability of the positive control was 14.6 ± 4.2 of the negative control. These were below the maximum acceptance values of 40% viability and SD of 18.
EPISKIN™ results: The percentage value for the OD of test substance NTO, linked to colour was 2.074% of the negative control with MTT (Table 1). As this value was below 5%, correction of the test substance OD values was not required.
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