Registration Dossier
Registration Dossier
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EC number: 226-970-7 | CAS number: 5580-57-4
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
'Yellow disazo condensation pigments' are with high probability acutely not harmful to aquatic invertebrates.
No toxic effects occur at the range of the substances solubility.
EC50 (48h) > 100 mg/L (nominal) for Daphnia magna (OECD 202, EU Method C.2)
Key value for chemical safety assessment
Additional information
The acute toxicity on aquatic
invertebrates was investigated for two ‘yellow disazo condensation
pigments’ (CAS 68516-73-4 and CAS 5580-57-4) with the waterflea
Daphnia magna as test species (CETA, 2011; CETA, 2001). Based on the
low water solubility of the test item, saturated stock solutions were
prepared and the tests were conducted as limit tests with a nominal
concentration of 100 mg/L. For one study (CAS 68516-73-4), which was
conducted under GLP conditions according to OECD guideline 202, the
stock solution was filtered through 0.45 µm filter (CETA, 2011). In
the other non-GLP study according to EU Method C.2 (CAS 5580-57-4),
the stock solution was used without filtration (CETA, 2001). Hence,
non-dissolved material was observed in the test vessels, which
disturbed the measurement of the oxygen content in the test solution.
However, in both studies the EC50 values after 48 hours of exposure
are above the 100 mg/L test item concentration.
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