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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed guideline-conform study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
Deviations:
not specified
Qualifier:
equivalent or similar to guideline
Guideline:
ISO 7827 (Evaluation in an Aqueous Medium of the "Ultimate" Aerobic Biodegradability of Organic Compounds - Method by Analysis of Dissolved Organic Carbon (DOC))
Deviations:
not specified
Qualifier:
equivalent or similar to guideline
Guideline:
other: 92/69IEWG (Teil CA-A),
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
3-nitro-p-toluic acid
EC Number:
202-549-3
EC Name:
3-nitro-p-toluic acid
Cas Number:
96-98-0
Molecular formula:
C8H7NO4
IUPAC Name:
4-methyl-3-nitrobenzoic acid
Details on test material:
purity: 99.4 % +- 0.6%

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Source: Domestic waste water treament plant Frankfurt am Main / Sindlingen
KInd of inoculum: centrifuged sediment from activated sludge was washed, centrifuged again and resuspended
Pre-treatment: 6 days aeration
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
55 mg/L
Based on:
test mat.
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
1 d
Parameter:
% degradation (DOC removal)
Value:
7.6
Sampling time:
11 d
Parameter:
% degradation (DOC removal)
Value:
8.7
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
day 7: 101.3 % biodegradation

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other: not readily biodegradable
Conclusions:
The substance is not readily biodegradable
Executive summary:

The substance was tested for ready biodegradation according to OECD guideline 301 A.

The rate of biodegradation was below 20% after 28 days.