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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
September - October 1976
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: NON GLP, no guideline reported.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976
Report Date:
1976

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Please refer to the Executive Summary for details on method.
GLP compliance:
no
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
not specified
Details on test material:
- Name of test material (as cited in study report): Parachlorbenzotrifluorid

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 121-148 g
- Housing: plastic cages on sawdust
- Fasting: no food since 16 hs before treatment
- Diet : ALTROMIN 1324 ad libitum
- Water: ad libitum


IN-LIFE DATES: From: 28 September To: 12 October

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
vegetable oil
Remarks:
sesam oil
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 25%

MAXIMUM DOSE VOLUME APPLIED: 10.000 mg/kg
Doses:
4000, 6300, 10000 mg/kg
No. of animals per sex per dose:
10 (males)
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs.
Statistics:
Probit analysis

Results and discussion

Preliminary study:
No
Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
5 546 mg/kg bw
Based on:
test mat.
95% CL:
> 4 575 - < 6 724
Mortality:
4000 mg/kg: 1/10 rat
6300 mg/kg: 8/10 rat
10000 mg/kg: 9/10 rat
Clinical signs:
convulsions, loss of balance,
Gross pathology:
whitish mucosa in the small intestine
Other findings:
No finding in macroscopic evaluation.

Applicant's summary and conclusion

Interpretation of results:
practically nontoxic
Remarks:
Migrated information Criteria used for interpretation of results: other: Handbook of Toxicology (1957), W.S. Spector
Conclusions:
The test item LD50 is 5546 mg/kg for male rats. The test item results practically non-toxic.
Executive summary:

After a fasting period of 16 days, the test item Parachlorbenzotrifluorid was administered by oral gavage to 10 male Wistar rats for each dose level (4000, 6300 and 10000 mg/kg b/w). The observed mortality 'til day 4 was as follows:

dose level 4000 mg/kg : 1/10 rats died

dose level 6300 mg/kg : 8/10 rats died

dose level 10000 mg/kg: 9/10 rats died

The registered clinical signs were: loss of balance and convulsions.

The LD50 was calculated by Probit analysis and was found to be 5546 mg/kg b/w.