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EC number: 257-856-5 | CAS number: 52334-81-3
Dermal absorption
Administrative data
- Endpoint:
- dermal absorption in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline Study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 428 (Skin Absorption: In Vitro Method)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-chloro-5-(trifluoromethyl)pyridine
- EC Number:
- 257-856-5
- EC Name:
- 2-chloro-5-(trifluoromethyl)pyridine
- Cas Number:
- 52334-81-3
- Molecular formula:
- C6H3ClF3N
- IUPAC Name:
- 2-chloro-5-(trifluoromethyl)pyridine
- Reference substance name:
- 2-chloro-5-(trifluoromethyl) pyridine
- IUPAC Name:
- 2-chloro-5-(trifluoromethyl) pyridine
- Test material form:
- solid: crystalline
- Details on test material:
- CTF is a white crystalline solid with a sweet odour and slightly basic in nature. Specific gravity 1.35g/ml at 72°C, boiling point 152°C. It is stable to light, heat, air and moisture and soluble in 50/50 v/v ethanol/water. The sample was 98.4% pure with the major impurities 3-chloro-5-trifluoromethyl pyridine (5-CTF): 0.47%, 2-chloro-3-trifluoromethyl pyridine (2-CTF): 0.44% and 3-trifluoromethyl pyridine (TF): 0.24%.
Test Substance Control Number: Y00846/013/001.
Constituent 1
Constituent 2
Administration / exposure
- Type of coverage:
- open
- Vehicle:
- acetone
Results and discussion
Any other information on results incl. tables
Results
After application to the skin membranes of CTF as a 2.5mg/ml solution in acetone (which was allowed to evaporate away) the mean rate of absorption of CTF through human epidermal membranes was:
0.0070 mg/cm2/hr (SEM ± 0.0011 : n=11)
When similarly applied to rat whole skin the mean rate of absorption was:
0.0062 mg/cm2/hr (SEM ± 0.00008 : n = 20)
When applied to separated rat epidermal membranes the mean rate of absorption of CTF was:
0.0053 mg/cm2/hr (SEM ± 0.00096 : n=7)
The mean damage ratio due to contact of CTF with the skin membranes was:
Human epidermal membranes: 4.25 (SEM ± 2.4 : n = 3)
Rat whole skin: 2.19 (SEM ± 0.12 : n = 4)
Separated rat epidermal membranes: 4.01 (SEM ± 0.87 : n = 7)
Contact with ethanol/water (50/50 v/v) alone produces a damage ratio of ~ 2.0
Applicant's summary and conclusion
- Conclusions:
- 2-chloro-5-(trifluoromethyl) pyridine (CTF) only penetrated both human and rat skin epidermal membranes slowly.
- Executive summary:
2-chloro-5-(trifluoromethyl) pyridine (CTF) was applied in acetone (2.5mg/ml) to prepared human and rat epidermal membranes in glass diffusion cells. The mean in vitro percutaneous absorption rate of CTF was
0.0070mg/cm2/hr (SEM ± 0.011 : n = 11) through human skin
0.0053mg/cm2/hr (SEM ± 0.0096 : n = 11) through rat skin
These results indicate that CTF penetrated both these skin types slowly.
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