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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Sponsor's identification: SR531
Description: pale yellow liquid
Batch number: KTE0864
Date received: 16 June 2010
Expiry date: 16 December 2010
Storage conditions: room temperature in the dark

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, predominantly domestic, non-adapted
Details on inoculum:
A mixed population of activated sewage sludge micro-organisms was obtained on
5 July 2010 from the aeration stage of the Severn Trent Water Pie sewage treatment
plant at Loughborough, Leicestershire, UK, which treats predominantly domestic
sewage.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
10 mg/L
Based on:
other: carbon
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
28
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
Sodium benzoate attained 72% degradation after 14 days 84% degradation after
28 days thereby confirming the suitability of the inoculum and test conditions.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
inherently biodegradable