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EC number: 687-538-5 | CAS number: 18423-20-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Reconstructed Human Epidermis (RHE) Test Method)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3,5,7-triaza-1-azoniatricyclo[3.3.1.13,7]decane nitrate
- EC Number:
- 687-538-5
- Cas Number:
- 18423-20-6
- Molecular formula:
- C6H13N5O3
- IUPAC Name:
- 3,5,7-triaza-1-azoniatricyclo[3.3.1.13,7]decane nitrate
Constituent 1
In vitro test system
- Test system:
- human skin model
- Source species:
- human
- Cell type:
- other: 0.38 cm2 RhE of normal human keratinocytes
- Cell source:
- foreskin from multiple donors
- Justification for test system used:
- EpiSkinTM is one out of the four validated test methods using commercially available RhE models included in the OECD TG Nº431. EpiSkinTM can be used for regulatory purposes for distinguishing between corrosive and non-corrosive substances and can moreover be used to support sub-categorisation of corrosive substances.
- Details on test system:
- Reconstructed Human Epidermis (RhE; EpiSkin-SM): This is a three-dimensional human skin model comprising a reconstructed epidermis with a
functional stratum corneum. Differentiated and stratified epidermis model comprises the main basal, supra basal, spinous and granular layers and a functional stratum corneum.
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- % tissue viability
- Remarks on result:
- other: The percentage of viability was determined to be 103.9%, 104.0% and 92.2%at the 3min, 1h and 4h time points, respectively, using living RhE inserts.
- Remarks:
- Therefore, the compound was classified as non-corrosive following the criteria of OECD 431, since the viability was ≥35% after 4 hours of exposure.
- Other effects / acceptance of results:
- The run carried out with the living RhE inserts met the predefined acceptance criteria, i.e., i) mean ODNC of the RhE inserts treated with the negative control was within the range 0.6-1.5 at 570 nm ± 30 nm; ii) the relative viability for the positive control at the 4h exposure time point expressed as % of the negative control was ≤20%; and iii) in the range of 20 -100% viability and for ODs≥0.3, the difference in the viability between the two RhE replicates did not exceed 30%.
For the test item Hexamine nitrate, the percentage of viability was determined to be 103.9%, 104.0% and 92.2% at the 3min, 1h and 4h time points, respectively, using living RhE inserts. Therefore, the compound can be classified as non-corrosive following the criteria of OECD 431, since the viability was ≥35% after 4 hours of exposure.
Applicant's summary and conclusion
- Interpretation of results:
- other:
- Conclusions:
- Taking into account the results obtained in the current study, it is concluded that, under the assayed experimental conditions and according to the OECD guideline for the Testing of Chemicals Nº. 431, the test item Hexamine Nitrate can be categorised as Non-Corrosive according to the United Nations Globally Harmonized System of Classification and Labelling of Chemicals.
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