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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Description of key information

NOEC (28 d and 56 d) = 1000 mg/kg dw for Eisenia fetida (OECD 222)

Key value for chemical safety assessment

Additional information

No experimental data evaluating the toxicity of methyl hexanoate (CAS No. 106-70-7) to soil macroorganisms are available.

Therefore, data from a structurally related category member, methyl laurate (CAS No. 111-82-0) are used as read-across in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5. Both substances are fatty esters esterified with methanol, differing only in the C-chain length of the fatty acid component: C6 (CAS No. 106-70-7) and C12 (CAS No. 111-82-0) respectively. Methyl laurate (C12 FA content > 80%) exhibits the highest toxicity to aquatic organisms (Daphnia and algae) throughout the whole SCAE Me category, with EC50 and NOEC values < 1 mg/L.Considering that, methyl laurate is used as worst-case read across substance in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5.

The toxicity of methyl laurate (CAS No. 111-82-0) to earthworms was evaluated by Winkelmann (2013) in a test according to OECD 222, under GLP conditions. Eisenia fetida was exposed to the test substance for 56 days at a concentration of 1000 mg/kg dw (limit test). Mortality and growth were evaluated after 28 days whereas reproduction was evaluated after 56 days. After the exposure period, no effects on earthworm’s survival, reproduction or growth were observed, leading to a NOEC = 1000 mg/kg dw.


Based on the results obtained for the structurally related category member (in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5), it can be concluded that methyl hexanoate will not exhibit toxicity to soil macroorganisms.