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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
other information
Study period:
13 Sept 2013 - 03 April 2014
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014
Report date:
2014

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
(3R,4S,5R,6R)-3,4,5-tris[(trimethylsilyl)oxy]-6-{[(trimethylsilyl)oxy]methyl}oxan-2-one
EC Number:
608-732-8
Cas Number:
32384-65-9
Molecular formula:
C18 H42 O6 Si4
IUPAC Name:
(3R,4S,5R,6R)-3,4,5-tris[(trimethylsilyl)oxy]-6-{[(trimethylsilyl)oxy]methyl}oxan-2-one
Test material form:
other: viscous liquid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
The necessary amount of BI 10773 TMS-Lactone for preparing the solutions was weighed on weighing scoops and transferred to volumetric flasks. Test medium (see 4.4) was added up to the bench mark and the solution was homogenised by shaking. The test solution volume was 50 mL per test vessel. Finely dispersed oily droplets were observed during preparation of test solutions, but not during the test. The test solutions were clear during the test, without any precipitation.
Concentration: 0.100, 0.320, 1.00, 3.20 and 10.0 mg/L

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Daphnia magna STRAUS, Clone V,
- Source: German Federal Environment Agency (UBA)
- Age at study initiation (mean and range, SD): max. 24 h
- Method of breeding: Daphnia magna was bred as single culture (one daphnid per 100 mL) in Elendt
M4 medium. The pH-value of the aerated water was within a range of 6.0 – 9.0.
The dissolved oxygen was above 60 % saturation and the total hardness 140 -
268 mg/L (as CaCO3).
- Feeding during test: single cell green algae
(Desmodesmus subspicatus, formerly Scenedesmus subspicatus) at least three
times a week.

Study design

Test type:
static
Water media type:
not specified
Total exposure duration:
48 h

Test conditions

Hardness:
14°dH ( 250mg/L as CaCo3)
Test temperature:
19.5 – 20.4 °C
pH:
7.67 – 7.90
Dissolved oxygen:
97 %
Nominal and measured concentrations:
Test concentrations: 0.100 mg/L - 10.0 mg/L

The analysed concentrations in the initial test medium samples were between 77
and 100 % of the nominal concentrations. During the test period the test item
concentrations decreased to 2 - 16 % of nominal.
Since the mean measured concentrations of TMS-Lactone were below 80 % of
nominal, the toxicological endpoints were evaluated using nominal and actual
concentrations of the test item, determined by calculating the geometric mean for
each concentration level.
Details on test conditions:
TEST SYSTEM
- Test vessel: glass beaker
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 100 mL filled with 50 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Justification for using less concentrations than requested by guideline: A flat dose-response relationship was observed in the range-finding test.
- Test concentrations: 0.100 mg/L - 10.0 mg/L
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
4 mg/L
Nominal / measured:
meas. (initial)
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.043 mg/L
Nominal / measured:
meas. (initial)
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.175 mg/L
Nominal / measured:
meas. (initial)
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.32 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.04 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Results with reference substance (positive control):
- Results with reference substance valid? Yes
The results indicate an EC50 (24 h) of the reference item potassium dichromate of
2.0 mg/L. Since the results fall within the historical data generated with the
reference item at the testing facility and are within the range of 0.6 - 2.1 mg/L recommended by the test guideline OECD 202, the daphnids were suitable for the determination of the toxicological effects of the test item.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
According to the results of the test, the EC50 (48 h) is 1.04 mg test item /L (nominal) corresponding to 0.175 mg test
item/L (actual). The EC0 (48 h) was observed at 0.320 mg test item /L (nominal) and 0.0425 mg test item/L (actual), the
EC100 (48 h) at 10.0 mg test item/L (nominal) corresponding to 4.00 mg test item/L (actual).