N,N-diethylaniline

Endpoint summary

Description of key information

Adsorption/desorption:

The adsorption coefficient Koc in soil and in sewage sludge of test chemical was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. The solutions of the test substance and reference substances were prepared in appropriate solvents. A test item solution was prepared by accurately pipetting 4 microlitre of test item and diluted with Acetonitrile upto 10 ml. Thus, the test solution concentration was 375.2 mg/l(calculated according to its density). The pH of test substance was 6.4. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were calculated. The graph was plotted between log Koc versus log k(Annex - 2).The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor. The reference substances Acetanilide, 4-chloroaniline, 4-methylaniline(p-Tolouidine), N-methylaniline, p-toluamide, Aniline, 2,5-Dichloroaniline, 4-nitrophenol, 2 - nitrophenol, 2-nitrobenzamide, 3-nitrobenzamide, Nitrobenzene, 4-Nitrobenzamide, 1 -naphthylamine, 1-naphthol, Direct Red 81, Benzoic acid methylester, Carbendazim, Benzoic acid phenylester, Xylene, Ethylbenzene, Toluene, Naphthalene, 1,2,3-trichlorobenzene, Pentachlorophenol, Phenol, N,N-dimethylbenzamide, 3,5-dinitrobenzamide, N-methylbenzamide, Benzamide, phenanthrene, DDT were chosen having Koc value range from 1.25 to 5.63.The Log Koc value of test chemical was determined to be 3.424 ± 0.000 dimensionless at 25°C.This log Koc value indicates that the substance has a moderate sorption to soil and sediment and therefore has slow migration potential to ground water.

Additional information

Adsorption/desorption:

Various experimental studies have been reviewed for test chemical for Adsorption endpoint and their results are summarized below.

The first study was reviewed from experimental study report in this report the adsorption coefficient Koc in soil and in sewage sludge of test chemical was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. The solutions of the test substance and reference substances were prepared in appropriate solvents. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were calculated. The graph was plotted between log Koc versus log k(Annex - 2).The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor. The reference substances Acetanilide, 4-chloroaniline, 4-methylaniline(p-Tolouidine), N-methylaniline, p-toluamide, Aniline, 2,5-Dichloroaniline, 4-nitrophenol, 2 - nitrophenol, 2-nitrobenzamide, 3-nitrobenzamide, Nitrobenzene, 4-Nitrobenzamide, 1-naphthylamine, 1-naphthol, Direct Red 81, Benzoic acid methylester, Carbendazim, Benzoic acid phenylester, Xylene, Ethylbenzene, Toluene, Naphthalene, 1,2,3-trichlorobenzene, Pentachlorophenol, Phenol, N,N-dimethylbenzamide, 3,5-dinitrobenzamide, N-methylbenzamide, Benzamide, phenanthrene, DDT were chosen having Koc value range from 1.25 to 5.63. The Log Koc value of test chemical was determined to be 3.424 ± 0.000 dimensionless at 25°C.This log Koc value indicates that the substance has a moderate sorption to soil and sediment and therefore has slow migration potential to ground water.

Next study reviewed from journal Chemosphere (1997) in this study an experimental was performed to determine adsorption coefficient o test chemical by using two different sludges from lab simulation in this study the test chemical solution is added to the sludge in centrifuge tubes and shaken. Thereafter the solution is centrifuged and the remaining concentration in the supernatant is measured.

The following modifications were made for sewage sludges:

1. 0.2 g dried sewage sludges were used for 50 ml test solution

2. for fresh sludges about 0.2 g (dry weight) were used in each test vessel

3. in addition to test solutions containing a 0.01 M CaC12 concentration, solutions of different water hardnesses were prepared by solving the desired concentrations of CaC12 in the test solution.

The log Koc value of test chemical was determined to be 1.68 and 1.76 dimensionless in two different sludges from lab simulation plant by using HPLC method. By considering log Koc value it is concluded that test chemical has low sorption to sludge and moderate migration potential to ground water.

 

Next study was reviewed from Ecotoxicology and Environmental Safety journal (1980) in this study the Koc value of test chemical was estimated by using equation taken from Kenaga and Goring (1978). The Koc value of test chemical was estimated to be 120 dimensionless (log Koc=2.079). By considering this log Koc value it is concluded that test chemical has low sorption to soil and sediment and therefore has moderate migration potential to ground water.

Last study was reviewed from authoritative database (HSDB, 2017) in this study the Koc value of test chemical was estimated by using a log Kow of 3.31and a regression-derived equation. The Koc of test chemical was estimated to be 1500 dimensionless log Koc= 3.1760. By considering this log Koc value of test chemical it is concluded that test chemical has moderate sorption to soil and sediments and therefore has slow migration potential to ground water.

By considering results of all the studies mentioned above it is concluded log Koc value of test chemical is in range of 1.68 – 3.4 dimensionless. This range log Koc value indicates that the substance has a low to moderate sorption to soil and sediment and therefore has moderate to slow migration potential to ground water.