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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

Based on the test results the 96-hour LC50 of 3,3,5-trimethylcyclohexanone for Zebrafish (Danio rerio) was determined to be higher than 100 mg test item/L based on nominal concentrations. The no observed effect concentration (NOEC) was determined to be 45 mg test item/L and the LOEC was determined to be 100 mg test item/L, both values also based on nominal concentrations.

Key value for chemical safety assessment

Additional information

In a 96-h acute toxicity study, Zebrafish (Danio rerio) were exposed to 3,3,5 -Trimehtylcyclohexanone at nominal concentrations of 0 (control), 4.3, 9.4, 21, 45, and 100 mg/L under static conditions.

The 96-h LC50was determined to be > 100 mg/L. The LOEC and NOEC values, based on sublethal effects, were 100 and 45 mg/L, respectively.  Sublethal effects of apathy, dark colouration, tumbling during swimming and fish mainly at the bottom were observed in the groups exposed to 100 mg/L after 2h until the end of the test.

Based on the results of this study, 3,3,5 -Trimehtylcyclohexanone would not be classified as toxic to Danio rerio in accordance with the GHS classification system.


This toxicity study is classified as acceptable and satisfies the guideline requirement for OECD 203 “Fish, Acute Toxicity Test”.


Results Synopsis


Test organism size/age (mean wet weight or length): 1.99 cm +/- 0.11 cm

Test Type (Static):

LC50(96 h):    > 100 mg/L                         95% C.I.: not determined

LC0(96 h):         100 mg/L

NOEC (96 h): 45 mg/L 

LOEC (96 h): 100 mg/L