Registration Dossier
Registration Dossier
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EC number: 231-974-7 | CAS number: 7783-00-8
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well performed study. Effect concentrations based on measured Se concentrations.
Data source
Reference
- Reference Type:
- other: manuscript
- Title:
- The Effects of Lesser Known Metals and One Organic to Fathead Minnows (Pimephales promelas) and Daphnia magna
- Author:
- Kimball G
- Year:
- 1 978
- Bibliographic source:
- Department of Entomology, Fisheries and Wildlife, University of Minnesota, Minneapolis, MN, 88pp
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Selenious acid
- EC Number:
- 231-974-7
- EC Name:
- Selenious acid
- Cas Number:
- 7783-00-8
- Molecular formula:
- H2O3Se
- IUPAC Name:
- selenous acid
- Details on test material:
- - Name of test material (as cited in study report): Selenious acid
- Molecular formula (if other than submission substance): H2SeO3
- Purity: reagent grade
- No further information on substance stated.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Samples for metal analysis were drawn into 15 ml tubes and acidified with 1 drop of 1.0 N HNO3.
- Frequency: 3 times weekly
Test solutions
- Vehicle:
- no
- Details on test solutions:
- see test conditions
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Obtained from a commercially available source (Aquatic Life Company, Minneapolis, USA)
- Neonates, 12 ± 12 h old
- Animals were fed but no information on type and amount of food was stated.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 28 d
Test conditions
- Hardness:
- no data (hard water)
- Test temperature:
- 20.4°C (average)
- pH:
- 8.47 (average)
- Dissolved oxygen:
- 7.43 mg/L (average)
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Control + 0.07-0.12-0.19-0.30-0.48-0.92 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL beaker, 50 mL fill volume
- Type of flow-through (e.g. peristaltic or proportional diluter): static renewal system
- Renewal rate of test solution (frequency/flow rate): three times a week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10 replicates
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Hard water from a well tapping the Jordan sandstone stratum underlying the Minneapolis-St. Paul metropolitan area. Iron was catalytically removed (0.1 mg/L).
- Alkalinity: 210 mg CaCO3/L (average)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 70 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- element
- Remarks:
- Se
- Basis for effect:
- reproduction
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 120 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- element
- Remarks:
- Se
- Basis for effect:
- reproduction
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 190 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- element
- Remarks:
- Se
- Basis for effect:
- mortality
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 300 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Remarks:
- Se
- Basis for effect:
- mortality
- Details on results:
- 28 d LC50 of 240 µg Se/L based on mortality.
- Reported statistics and error estimates:
- The data was examined by one-way analysis of variance with treatment differences separated by Dunnett's procedure (one-tailed) at alpha=0.05.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Klimisch 2 study on the chronic toxicity of selenium to Daphnia magna. The study was conducted according to a procedure similar to standard procedures for reproduction toxicity tests with cladocerans. The 28-day NOEC, LOEC and LC50 for mortality were 190, 300, and 240 µg Se/L, respectively. The 28-day NOEC and LOEC for reproduction were 70 and 120 µg Se/L, respectively.
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