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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well performed study. Conducted according to US EPA guidelines for acute testing. LC50 values based on measured Se concentrations.

Data source

Reference
Reference Type:
publication
Title:
Comparative Toxicity of Selenate, Selenite, Seleno-DL-Methionine and Seleno-DL-Cystine to Daphnia Magna
Author:
Maier KJ, Foe CG, Knight AW
Year:
1993
Bibliographic source:
Environmental Toxicology and Chemistry 12: 755-763

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: EPA-660/3-75-009: Methods for Acute Toxicity Tests with Fish, Macroinvertebrates, and Amphibians
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium selenite
EC Number:
233-267-9
EC Name:
Sodium selenite
Cas Number:
10102-18-8
Molecular formula:
H2O3Se.2Na
IUPAC Name:
disodium selenite
Details on test material:
- Name of test material (as cited in study report): Selenite
- Molecular formula (if other than submission substance): Na2SeO3
- Molecular weight (if other than submission substance): 173 g/mol
- Analytical purity: no data
- Source: Sigma Chemical Co., St. Louis, MO, USA

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Frequency: At the start and end of exposure

Test solutions

Vehicle:
no
Details on test solutions:
see test conditions

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: Laboratory stock
- Age at study initiation: Neonates
- Feeding during test: No

ACCLIMATION
- Acclimation period: no data

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
as measured ± 3% mg/L as CaCO3
Test temperature:
20 ± 0.1 °C
pH:
8.2 ± 0.1
Dissolved oxygen:
8.6 ± 0.2 mg/L
Salinity:
Not applicable
Nominal and measured concentrations:
as calculated ± 5% mg Se/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 500 ml acid-washed glass jars with 100 mL of test medium
- Renewal rate of test solution (frequency/flow rate): 95% of solution replaced at 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted, moderately hard, fresh water that contained salt concentrations twice those in EPA soft fresh water. The initial water was reverse-osmosis-treated water that met EPA specifications, to which 96 mg/L NaHCO3, 60 mg/L CaSO4.2H2O, 60 mg/L MgSO4, and 4 mg/L KCl (all reagent grade) were added.
- Sulfate: 81.5 mg/L

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
550 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Se
Basis for effect:
mortality
Reported statistics and error estimates:
LC50 values were interpolated by probit analysis, using the SAS statistical program.

Applicant's summary and conclusion

Conclusions:
Reliable (Klimisch 1) study on toxicity of sodium selenite to Daphnia magna. The 48-h LC50 value was 550 µg Se/L.

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