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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well performed study. Effect concentrations based on measured Se concentrations.

Data source

Reference
Reference Type:
publication
Title:
Acute toxicity of selenium dioxide to freshwater fishes
Author:
Cardwell RD, Foreman DG, Payne DR, Wilbur DJ
Year:
1976
Bibliographic source:
Archives of Environmental Contamination and Toxicology 4: 129-144

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EPA OPP 72-1 (Fish Acute Toxicity Test)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Selenium dioxide
EC Number:
231-194-7
EC Name:
Selenium dioxide
Cas Number:
7446-08-4
Molecular formula:
O2Se
IUPAC Name:
oxoselane oxide
Details on test material:
- Name of test material (as cited in study report): Selenium dioxide
- Molecular formula (if other than submission substance): SeO2
- Molecular weight (if other than submission substance): 111 g/mol
- Analytical purity: 99.5%
- Source: Research Organic/Inorganic Chemical Corp.

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
-Frequency: Three to four times during bioassay
- Sampling method: Upon collection, samples of water were filtered through Whatman No. 1 paper to remove debris and acidified to insure toxicant stability

Test solutions

Vehicle:
no
Details on test solutions:
see test conditions

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name: Fathead minnow
- Source: Cultured in laboratory from brood stock obtained from US EPA's National Water Quality Laboratory in Duluth, Minnesota, USA
- Age at study initiation (mean and range, SD): 1 day (yolk sac fry) or 3 months (juveniles)
- Length at study initiation: 5 mm (yolk sac fry) of 20.7 mm (juveniles)
- Weight at study initiation: 0.085 g (juveniles)
- Feeding during test: No

ACCLIMATION
- Acclimation period: 3 days prior to exposure
- Fish were not fed during the acclimation period

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
151 mg /L as CaCO3
Test temperature:
24.7 ± 0.4 °C
pH:
7.80 ± 0.09
Dissolved oxygen:
7.1 ± 0.6 mg/L
Salinity:
not applicable
Nominal and measured concentrations:
Control + 5 exposure concentrations up to 54 mg SeO2/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 30.5x30.5x30.5 cm glass chambers containing 16.5 L of test solution
- Type of flow-through (e.g. peristaltic or proportional diluter): 2-L proportional diluters
- Renewal rate of test solution (frequency/flow rate): 90% renewal in 9 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: no data
- Alkalinity: 167 ± 4 mg/L as CaCO3
- Sulfate: 8.6 mg/L

OTHER TEST CONDITIONS
- Photoperiod: 12:12 (light:dark)
- Light intensity: 1010 lux

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2 060 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Se
Basis for effect:
mortality (fish)
Remarks on result:
other: 1-d-old yolk sac fry
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
5 190 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Se
Basis for effect:
mortality (fish)
Remarks on result:
other: 3-month-old juveniles
Reported statistics and error estimates:
Probit analysis or Litchfield-Wilcoxon in case of only one partial kill.

Applicant's summary and conclusion

Conclusions:
Reliable (Klimisch 1) study on the toxicity of selenium dioxide to Pimephales promelas (fathead minnow). The 96-h LC50 values for 1-d-old yolk sac fry and 3-month-old juveniles were 2060 and 5190 µg Se/L, respectively.

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