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In short-term toxicity tests of lactic acid in three different fish species, mortality was observed resulting in estimated LC50 values ranging between 130 and > 320 mg/L (nominal), or 130 to 195 mg/L (mean measured concentration). The effects can be attributed to a pH reduction induced by lactic acid. Lactate as such is considered not exert any systemic effects on fish in the tested concentration range of up to 560 mg/L.

Furthermore, in several short-term toxicity tests of lactic acid in water fleas, immobilisation was observed resulting in estimated EC50 values ranging between 130 and 750 mg/L (nominal), which can again be attributed to a pH effect. Lactate as such is not considered to exert any systemic effects on daphnids in the tested concentration range of up to 750 mg/L.

Lactic acid is practically non-toxic to algae, with a NOEC > 533 mg/L (measured TWA).

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