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Toxicity to soil microorganisms

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Description of key information

28d-EC10 of 130 mg/kg a.i. dw and 28d-EC50 of 190 mg/kg a.i. have been determined from an OECD 216 with 3-hydroxy-1,1-dimethylbutyl 2-ethyl-2-methylheptaneperoxoate.

Key value for chemical safety assessment

Short-term EC50 for soil microorganisms:
190 mg/kg soil dw
Long-term EC10 or NOEC for soil microorganisms:
130 mg/kg soil dw

Additional information

One valid experiment has been carried out according to OECD 216 guideline and GLP requirements (Bangert, 2015) in order to assess the effects of 3-hydroxy-1,1-dimethylbutyl 2-ethyl-2-methylheptaneperoxoate on the activity of the soil microflora (nitrogen transformation).

The study was performed using 5 concentrations 100 / 180 / 320 / 560 / 1000 mg/kg dry soil.

Sieved soil was exposed to the test item for 28 days. After 0 days (start of the test) and 28 days, the nitrate content of the soil of each treatment was determined. A blank control was tested.

The following concentrations showed inhibition of Nitrate production compared to the control: 1000 / 560 / 320 mg/kg

The following results were determined: 28d-EC50 = 190 mg/kg a.i. and 28d-EC10 = 130 mg/kg a.i..

The relative standard deviation between the replicates of the blank control was 1.2 %, indicating the small difference between the replicates. No observations were made which might cause doubts concerning the validity of the study outcome.

The result of the test can be considered valid.