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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
413-890-4
EC Name:
-
Cas Number:
82469-79-2
Molecular formula:
C28H50O8
IUPAC Name:
1,2,3-trihexyl 2-(butanoyloxy)propane-1,2,3-tricarboxylate

Study design

Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Reference substance
Reference substance:
other: Sodium Benzoate (10 mgC/L) & Hexadecane (10 mgC/L)

Results and discussion

% Degradation
Parameter:
% degradation (inorg. C analysis)
Value:
4
Sampling time:
28 d
Details on results:
Points of degradation plot (test substance):
0 % degradation after 2 d
0 % degradation after 4 d
0 % degradation after 6 d
0 % degradation after 8 d
1 % degradation after 10 d
1 % degradation after 13 d
1 % degradation after 20 d
1 % degradation after 22 d
4 % degradation after 28 d
13 % degradation after 34 d
24 % degradation after 38 d
36 % degradation after 43 d
47 % degradation after 50 d
54 % degradation after 57 d
60 % degradation after 64 d
66 % degradation after 71 d
70 % degradation after 78 d
74 % degradation after 85 d

BOD5 / COD results

Results with reference substance:
Points of degradation plot (reference substance):
32 % degradation after 2 d
48 % degradation after 4 d
61 % degradation after 6 d
68 % degradation after 8 d
71 % degradation after 10 d
74 % degradation after 13 d
78 % degradation after 20 d
78 % degradation after 22 d
80 % degradation after 28 d
81 % degradation after 34 d
81 % degradation after 38 d
81 % degradation after 43 d
81 % degradation after 50 d
81 % degradation after 57 d

Applicant's summary and conclusion

Interpretation of results:
other: not readily biodegradable
Conclusions:
Citroflex B-6 cannot be considered readily biodegradable, but is ultimately biodegraded in the presence of a weak inoculum at concentrations above its limit of water solubility following extended incubation.