Hydrazinium, 2-(3-methoxy-3-oxopropyl)-1,1,1-trimethyl-, bromide (1:1)

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08.10. - 17.10.2007.

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

other: Body responsible for the test

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

463-070-5

EC Name:

-

Cas Number:

106966-25-0

Molecular formula:

C7H17N2O2Br

IUPAC Name:

2-(3-methoxy-3-oxopropyl)-1,1,1-trimethylhydrazinium bromide

Test solutions

Details on test solutions:

The test substance is soluble in water in concentration needed for the test. The stock solution was prepared by dissolution of 2.5 g of test substance in 500 mL deionised water on the day of testing.
In the pleminary test the concentrations were in range of 46-1000 mg/L with a geometric progression factor of 2.2.
In the main test the concentrations were in range of 100-1000 mg/L with geometric progression factor of 1.8.

Test organisms

Test organisms (species):

activated sludge

Details on inoculum:

The activated sludge was collected two days before the day of testing.
After the sample collection the sludge was washed with potable water for 0.5 hour and subsequently decanted for 0.5 hour. This procedure was
repeated three times in total. Further the sludge was modified by addition of 50 mL of cultivation medium per 2 L of diluted sludge suspension at
permanent aeration till the day of test. The dry weight was determined from 10 mL of sludge suspension after 0.5 hour sedimentation.
Before the test the sludge was suspended in water up to concentration about 4000 mg of sludge dry weight per litre. The pH adjustment to 6.0 was
carried out. In that way modified sludge suspension was aerated untill the use.

Study design

Test type:

other: aerobic

Total exposure duration:

3 h

Test conditions

Test temperature:

22 ± 2 degrre of Celsius

pH:

7-8

Dissolved oxygen:

Oxygen consumption in the main test at the highest concentration (1000 mg/L) of test substance: ~ 8.58 mg/L

Nominal and measured concentrations:

Concentrations of the test substance: 100, 180, 320, 560, 1000 mg/L

Details on test conditions:

Lighting: daily
Stirring and aeration: with filtered pressured air, airflow rate 0.8 L/min

Results and discussion

Effect concentrations

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate