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1-((2-Butyloctyloxymethyl)-2-(3,4-dihydro-isoquinolinium-2-yl)ethyl)sulfate was tested in a bioconcentration test to Zebrafish (Danio rerio) over an uptake period of 14 days followed by a depuration period of 16 days according to the US EPA Guideline, OPPTS 850.1730, the OECD-Guideline 305, 1996 and the MITI-Guideline to the Testing Methods for New Chemical. The fish were exposed to the test concentrations 1.0 and 10 µg/L in a flow-through-system.14C-radio labaled test substance was used.

During the exposure to the test substance no toxic effects like mortality or changes in behavior or appearance were observed in the test organisms.

During the depuration phase the half-life time for the test substance in fish was 1.7 days in the low and 0.6 days in the high concentration. Approximately 90% of the steady state concentration of the test substance was excreted after 5.6 days in the low concentration and after 1.8 days in the high concentration. This indicates a fast depuration of the test substance from the organism.

The concentration in fish reached steady state within 10 days in both concentration groups.

Based on the steady state concentrations the bioconcentration factor BCFss determined in the two concentration groups was 10.8 in the lower concentration and 8.6 in the higher concentration. The uptake curve of both test groups was similar. The mean bioconcentration factor was 9.7.

Based on kinetic modeling the bioconcentration factor BCFk was 9.1 in the lower and 7.8 in the higher concentration. The mean bioconcentration factor was 8.5.

In conclusion the bioconcentration factor for 1-((2-Butyloctyloxymethyl)-2-(3,4-dihydro-isoquinolinium-2-yl)ethyl)sulfate was 9.1 based on the mean of BCFss and BCFk. This value is based on total radioactivity in the whole fish on wet weight basis.