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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

Triquat Monomer has no acute toxic effects on zebra fish up to the concentration of 100 mg/L (corrected for the water content(corrected for the water content, corresponding to 197 mg test item/L) under the present test conditions.

Key value for chemical safety assessment

Additional information

One reliable key study is available for this endpoint. The acute toxicity of the test item Triquat Monomer to zebra fish (Brachydanio rerio) was determined in a 96-hour static test according to the EU Commission Directive 92/69/EEC, Part C.1, the Commission Regulation (EC) No 440/2008, Part C.1 and the OECD Guideline for Testing of Chemicals No. 203 (1992). The validity criteria were met and the study has been performed in accordance with GLP.

 

A limit test was performed in accordance with the guidelines to demonstrate that the test item has no toxic effect on the test organisms at the concentration of 100 mg/L(corrected for the water content, corresponding to 197 mg test item/L). Thus, the only concentration tested was nominal 100 mg/L (corrected for the water content). Additionally, a control was tested in parallel.

 

As the test item is known to adsorb onto glass, the aquarium with the single test item concentration of 100 mg/L(corrected for the water content) was equilibrated for 72 hours before the test start to allow for correct dosing. The preparation of the test media was based on the OECD Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures, 2000.

 

The analytically determined concentrations of the test item Triquat Monomer in the test medium varied in the range from 102 to 107% of the nominal value. Thus, the correct dosing of the test item was confirmed. Furthermore, it has been demonstrated that the test item Triquat Monomer was stable under the conditions of the test during the test period of 96 hours. Therefore, all reported biological results are related to the nominal concentrations of the test item.

 

In the control and at the test concentration of 100 mg/L (corrected for the water content) no mortality or other visible abnormalities were determined at the test fish during the test period of 96 hours.

 

The summary of the biological results (based on test item concentration corrected for the water content) is as follows:

96h-LC50 > 100 mg/L

96h-NOEC >= 100 mg/L

96h-LOEC > 100 mg/L