Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

toxicity to aquatic algae and cyanobacteria
Adequacy of study:
other information

Data source

Reference Type:
other: Body responsible for the test

Materials and methods

Test guideline
according to guideline
other: Directive 92/69/EEC, C.3 OECD 201 (1984) ISO 8692 (1989) Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.
GLP compliance:

Test solutions

Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: As in addition stability in test medium was unknown, maximum
solubility was achieved by ultrasonic treatment (10-13
minutes) and a 10-minute stirring period of a supersaturated
dispersion of 100 mg/l followed by a subsequent filtration
through a 0.45 µm filter (Schleicher and Schuell, RC55). The
lower test concentrations for the range-finding test were
prepared by subsequent dilution of the filtrate in test
medium. The final test solutions were all clear and
colourless. Note that the control received similar
treatment, i.e. was also filtered.

Test organisms

Test organisms (species):
other: Selenastrum capricornutum, strain: CCAP278/4

Study design

Limit test:
Total exposure duration:
72 h

Test conditions

24 mg CaCO3/L

Results and discussion

Effect concentrationsopen allclose all
72 h
Dose descriptor:
Effect conc.:
> 4.6 mg/L
Basis for effect:
growth rate
72 h
Dose descriptor:
Effect conc.:
4.6 mg/L
Basis for effect:
growth rate
Details on results:
%Concentration loss over test: 61 ...

Applicant's summary and conclusion