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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted according to OECD Guideline under GLP conditions.
Qualifier:
according to
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
yes
Details on preparation and application of test substrate:
60 earthworms (six replicates of 10 worms) were exposed to a single concentration of 1000 mg/kg of soil for a period of 14 days at a temperature of 19 to 23C. The number of mortalities were determined after 7 and 14 days exposure.
Vehicle: tetrahydrofuran
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
60 earthworms (six replicates of 10 worms). Earthworms were obtained from the Original Organics Limited and maintained in 20 litre plastic trays at a temperature of 21C to 22C on a substrate consisting of 50% by volume of top soil and 50% by volume of animal manure. Transferred 24 hours prior to the start of the test to the artificial soil (without test substance).
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
14 d
Test temperature:
19 C to 23 C
pH:
Day 0
Control: 6.4 - 6.5; solvent controls: 6.4 - 6.5; 1000 mg/kg (nominal) - 6.3 - 6.4
Day 14
Control: 6.4 - 6.5; solvent controls: 6.3 - 6.4; 1000 mg/kg (nominal) - 5.5 - 5.8
Moisture:
See table below. Recorded at start and study termination.
Details on test conditions:
Recorded daily: Room Temperature between 19C - 23C; Light intensity (LUX) - 709 - 776
Nominal and measured concentrations:
1000 mg/kg
Reference substance (positive control):
yes
Remarks:
chloroacetamide
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
No mortalities or sublethal effects were observed.
Results with reference substance (positive control):
Within normal limits
Reported statistics and error estimates:
Earthworm data weight data was performed using Bartlett's test for homogeneity of variance and a Students t-test. All statistical analyses were performed using the SAS computer software package (SAS 1999 - 2001).
Validity criteria fulfilled:
yes
Conclusions:
The 14-day NOEC and LC50 of Molyvan 855 in earthworms (Eisenia foetida) is > 1000 mg/kg soil dw.

Description of key information

The 14-day NOEC and LC50 of Molyvan 855 in earthworms (Eisenia foetida) is > 1000 mg/kg soil dw.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:
1 000 mg/kg soil dw

Additional information

Key study

In an OECD 207 study conducted under GLP conditions, the 14 -day NOEC and LC50 of Molyvan 855 for earthworms (Eisenia foetida) is > 1000 mg/kg soil dw (Harlan Laboratories, 2011).