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EC number: 240-994-5 | CAS number: 16926-87-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07-09 August 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-hydroxy-3-phenoxypropyl methacrylate
- EC Number:
- 240-994-5
- EC Name:
- 2-hydroxy-3-phenoxypropyl methacrylate
- Cas Number:
- 16926-87-7
- Molecular formula:
- C13H16O4
- IUPAC Name:
- 2-hydroxy-3-phenoxypropyl 2-methylprop-2-enoate
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 171512
- Expiration date of the lot/batch: 23 December 2019
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Controlled room temperature (15-25ºC, below 70RH%), protected from humidity (tigh closed container)
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Final dilution of a dissolved solid, stock liquid or gel: Stock solution 100.00 mg/L
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- Analytical measurement was performed at the applied test concentration levels and from the control at the beginning and at the end of the experiment. The samples were analysed by HPLC system with UV detection method.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: Untreated control - the dilution water (ISO-medium) was used without addition of the test item. Reference control - Potassium dichromate
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): ISO-medium (according to OECD 202)
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): Stock solution - 100.00 mg/L
Final soltions (nominal) - 6.25, 12.5, 25.0, 50.0, 100.00 mg/L
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain/clone: Daphnia Magna
- Age at study initiation (mean and range, SD): <24 hours at the begining of the test
- Source: Istvan Szent University
ACCLIMATION
- Acclimation period: No acclimatization because the water used was similar to the culture water
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- The reconstituted water (ISO medium) had a total hardness of 245 mg/L (as CaCO3).
- Test temperature:
- The water temperature was measured at the start of the experiment and 24-hour intervals thereafter in each test vessel at all concentration levels. The test temperature was in the range of 20.2 – 20.7°C measured in the test vessels.
The additionally measured temperature in the climate chamber was between 19.7 and 21.0ºC. - pH:
- The pH of the test solution was not adjusted and not varied by more than 1.5 units in any one test. The pH was measured at the start and at the end of the experiment in each test vessel at all concentration levels and was in the range of 7.50 – 7.72.
- Dissolved oxygen:
- The dissolved oxygen concentration was measured in each test vessel at all concentration levels at the start and at the end of the test and was in the range of 8.1 – 8.6 mg/L.
- Nominal and measured concentrations:
- Nominal: 6.25, 12.5, 25.0, 50.0, 100.00 mg/L
Measured: 6.67, 13.20, 25.75, 51.25, 102.50 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers
- Type (delete if not applicable): open
RANGE-FINDING STUDY
- Test concentrations: 0.1, 1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: Because significant immobility was observed at the highest concentration level (100.0 mg/L) during the preliminary range-finding test, five test concentrations in a geometric series (using factor 2.0) and one control were used in the main test under static conditions (based on analytical results indicated that a static test was feasible). - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Twenty animals, divided into four groups (glass beaker) of five animals each were used at the test concentrations and for the control as well. The 24 and 48 hours EC50 values of the test item could not be calculated due to the slight effects observed. Consequently, the 24 and 48 hours EC50 and the 48h EC100 values of the test item were determined directly from the raw data.For the determination of the LOEC and NOEC, the immobilization at the test concentrations was tested on significant differences to the control values by Dunnett’s Test using TOXSTAT software. All validity criteria were met during this study.
- Reported statistics and error estimates:
- The 24 and 48 hours EC50 values of the test item could not be calculated due to the slight effects observed. Consequently, the 24 and 48 hours EC50 and the 48h EC100 values of the test item were determined directly from the raw data.For the determination of the LOEC and NOEC, the immobilization at the test concentrations was tested on significant differences to the control values by Dunnett’s Test using TOXSTAT software.
Any other information on results incl. tables
Validity
There was no immobilization in the control group and the dissolved oxygen concentration at the end of the test in control and test vessels was more than 3 mg/L.
All validity criteria were within acceptable limits and therefore the study is considered as valid.
Concentration of the test item
The following nominal concentrations were tested: 6.25, 12.5., 25.0, 50.0 and 100.0 mg/L.
Test concentrations were analytically determined at the start and at the end of the experiment. The corresponding measured geometric mean test item concentrations were: 6.67, 13.20, 25.75, 51.25 and 102.50 mg/L.
As the analytically measured concentrations deviated not more than 20 per cent from the nominal, the biological results are related to the nominal test item concentrations.
Analytically measured concentrations are shown in Table 3.
Table 3: Calculation of exposure concentrations
Nominal Concentration (mg/L) | Measured concentrations (mg/L) | Geometric mean | |
0 h/start | 48h/end | ||
Control | n.d. | n.d. | - |
6.25 | 6.58 | 6.76 | 6.67 |
12.5 | 13.1 | 13.3 | 13.20 |
25.0 | 26.1 | 25.4 | 25.75 |
50.0 | 51.6 | 50.9 | 51.25 |
100.0 | 102.7 | 102.3 | 102.50 |
n.d. - not detected
IMMOBILISATION
The number of immobilised animals and the percentage of immobility were determined at the 24th and 48th hour (Table 4).
In addition to immobility, no abnormal behaviour or appearance of test animals was detected.
Table 4: Number and percentage of immobilised animals
Nominal Concentration (mg/L) | Number of treated animals | Immobilised animals | |||
24 hours | 48 hours | ||||
number | percent | number | prcent | ||
Control 6.25 12.5 25.0 50.0 100.0 |
20 20 20 20 20 20 |
0 0 0 0 0 0 |
0 0 0 0 0 0 |
0 0 0 0 0 3* |
0 0 0 0 0 15 |
*: statistically significantly different compared to the control values (Dunnett’s Test; a= 0.05)
Data of immobility in each test vessel are detailed in Appendix 2.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Acute toxicity of PHPM was assessed with Acute immobilisation test on Daphnia magna, over an exposure period of 48 hours in a static system.
All validity criteria were met during this study.
Under the conditions of this Daphnia magna acute immobilisation study the observed endpoints for the effect of PHPM were the followings:
The 24h EC50 value: > 100.0 mg/L (nominal)
The 48h EC50 value: > 100.0 mg/L (nominal)
The 48h EC100 value: > 100.0 mg/L (nominal)
The 48h No-Observed Effect Concentration (NOEC): 50.0 mg/L (nominal)
The 48h Lowest Observed Effect Concentration (LOEC): 100.0 mg/L (nominal) - Executive summary:
Acute toxicity of PHPM on Daphnia magna was assessed in an acute immobilisation test, over an exposure period of 48 hours in a static test system.
Because significant immobility was observed at the highest examined concentration level during the preliminary range-finding test, five test concentrations in a geometric series (factor 2.0) and one control group were tested in the definitive test under static conditions.
The test concentrations were analytically determined at the start and at the end of the experiment.
The nominal concentrations of test item used in the main experiment were: 6.25, 12.5, 25.0, 50.0 and 100.0 mg/L.
The corresponding measured geometric mean test item concentrations were: 6.67, 13.20, 25.75, 51.25 and 102.50 mg/L.
As the measured concentrations deviated not more than 20 per cent from the nominal in all cases, biological results are based on thenominal test item concentrations.
Twenty animals, divided into four groups (glass beaker) of five animals each were used at the test concentrations and for the control as well.
The 24 and 48 hours EC50 values of the test item could not be calculated due to the slight effects observed. Consequently, the 24 and 48 hours EC50 and the 48h EC100 values of the test item were determined directly from the raw data.
For the determination of the LOEC and NOEC, the immobilization at the test concentrations was tested on significant differences to the control values by Dunnett’s Test using TOXSTAT software.
All validity criteria were met during this study.
Under the conditions of this Daphnia magna acute immobilisation study the observed endpoints for the effect of PHPM were the followings:
The 24h EC50value: > 100.0 mg/L (nominal)
The 48h EC50value: > 100.0 mg/L (nominal)
The 48h EC100value: > 100.0 mg/L (nominal)
The 48h No-Observed Effect Concentration (NOEC): 50.0 mg/L (nominal)
The 48h Lowest Observed Effect Concentration (LOEC): 100.0 mg/L (nominal)
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