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EC number: 477-470-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007-10-18 to 2007-11-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- certified by "Ministerium für Umwelt und Naturschutz, Landwirtschaft und Verbraucherschutz des Landes Nordrhein-Westfalen", Germany
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Aeration tank of a wastewater plant treating
predominantly domestic sewage (Wupper area water authority, STP Odenthal, Germany)
- Laboratory culture: no
- Storage conditions: The inoculum was stored at room temperature under continuous shaking with aeration until use for max. 3 days.
- Preparation of inoculum for exposure: The sludge was washed twice by adding mineral
medium, centrifuging (10 min at 2000 rpm and 20 °C) and decanting off the supernatant. 1.354 g of the wet sludge was dried in order to calculate the amount of wet sludge to achieve a
concentration of activated sludge of 3 g/L (dry wet) suspended solids. The calculated amount of sludge was first dissolved in mineral medium and then filled up to a defined end volume
- Concentration of inoculum: 30 mg/L ss - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 22 ± 1 °C
- pH: 7.4 to 7.9
- pH adjusted: no
- Aeration of dilution water: yes, before test start
- Suspended solids concentration: 30 mg/L ss
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: closed flasks
- Number of culture flasks/concentration: 2 replicates for test item, 3 flasks as blanks, 2 flasks for reference compound (sodium benzoate), 1 toxicity control
- Test apparatus: Oxi Top System (WTW)
- Test volume: 250 mL
- Chemical analysis and measuring equipment:
NO3-N/NO2-N: Determination of nitrite nitrogen and nitrate nitrogen and the sum of both by flow analysis (CFA and FIA), Standard: EN ISO 13395, Test apparatus : Continuous Flow Analyser SKALAR SAN Plus System
COD: Determination of Chemical Oxygen Demand of solids and substances which are not water-soluble (COD), Calibration Standard: Potassium hydrogen phthalate, Test apparatus : Behrotest / Mettler DL 70 ES with Rondo 60 and spectrometric detection
- Test performed in closed vessels: yes
SAMPLING
- Sampling frequency: daily
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 3 flasks
- Abiotic sterile control: no
- Toxicity control: yes, 1 flask
- Other: Reference substance: yes, 2 flasks
STATISTICAL METHODS: - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 48.8
- Sampling time:
- 28 d
- Details on results:
- Within 28 days, a degradation of 48.8 % was determined for the test substance.
- Results with reference substance:
- The reference compound sodium benzoate showed 86.3 % degradation after 14 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- The reference compound reached the level for ready biodegradability within 14 days. No toxicity of the test item was observed in the toxicity control. The oxygen uptake of the inoculum blank was ≤ 60 mg/L.
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The study indicates that the test substance is not readily biodegradable (48.8 % after 28 d (O2 consumption), OECD 301F).
- Executive summary:
A study was performed according OECD 301F to assess the ready biodegradability of Beta 30. The study was conducted in accordance with the Council Directive 92/69/EEC Method C.4-D “Manometric Respirometry Test“ (1992). This test method is in all essential parts identical with OECD Guideline 301 F.
A solution of Beta 30 in a mineral medium was inoculated and incubated for 28 d under aerobic conditions. During this period, degradation was followed by BOD determinations.
Beta 30 showed:
40.6 % degradation after 7 days
45.8 % degradation after 14 days
47.9 % degradation after 21 days
48.8 % degradation after 28 days
Therefore, Beta 30 is considered to be “Not Readily Biodegradable“.
The reference compound sodium benzoate showed 86.3 % degradation after 14 days.
Reference
Table 1:
Study Day | Test substance, mean of 2 replicates [%] | Reference substance, mean of 2 replicates [%] | Toxicity control [%] |
0 | - | - | - |
7 | 40.6 | 80.1 | 61.6 |
14 | 45.8 | 86.3 | 68.7 |
21 | 47.9 | 88.5 | 71.2 |
28 | 48.8 | 89.9 | 71.2 |
The toxicity control showed that the used concentrations of the test item did not show toxic effects to bacteria.
Description of key information
Not readily biodegradable: 48.8 % after 28 days (O2 consumption, OECD 301F)
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
An experimental study was performed according to the OECD 301F (GLP). Activated sludge from a domestic sewage treatment plant was used as inoculum. 48.8 % degradation of the test substance was recorded based on O2-consumption after 28 d. Thus, the test substance is not readily biodegradable according to the OECD criteria. A toxicity control containing both, reference substance and test substance, did not indicate inhibitory effects of the test substance to the inoculum (68.7 % degradation after 14 d; trigger value of 25% biodegradation in the toxicity control was reached).
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