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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- effects on growth of green algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 31 July 2020
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- OECD Guideline 201 "Water Quality - Freshwater Algae (Unicellular Green Algae) Growth Inhibition Test" (May 2012).
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- OECD 201 GLP test is in progress.
- Analytical monitoring:
- yes
- Remarks:
- An organic carbon measurement was performed for the stock solution and two test concentrations in order to estimate the amount of test element present in the test solutions and its stability throughout the period of time exposure.
- Details on sampling:
- The test element considered to be slightly soluble in water, solubilization tests were carried out in order to optimize the dissolution.
These tests were carried out in different media (ultrapure water, algae test media) and the solubilization rate was estimated on the amount of organic carbon measured in the aqueous phase.
The various tests carried out to solubilize the test item showed very similar results.
Indeed, whatever the stirring time, the test medium used, the percentage of solubilized organic carbon was between 75 and 78% compared to the carbon content measured in the test item.
At the highest concentration tested, 500 mg/L, the percentage of solubilized organic carbon was identical to the other test solutions. However, at this concentration, the texture of the prepared solution was not suitable for the ecotoxicity tests. Thus, the maximum concentration tested was limited to 200 mg/L.
The TOX20022 being composed of 80 to 100% lipoamino acid, the follow-up of the solubilized organic carbon content shows that a significant part of the element is present in the test solutions but does not allow to distinguish the part of lipoamino acid dissolved from to the other constituents potentially present in the tested product (which may represent up to 20% of the product).
In addition, it seemed that a part of the test item was absorbed, among other things, on the bar magnet used to perform the magnetic stirring.
In the remainder of the report, the test concentrations will therefore be presented in nominal concentrations of TOX20022. - Vehicle:
- no
- Details on test solutions:
- Range of nominal concentrations (in mg/L): 2.90 - 6.40 - 14.1 - 31.0 - 68.2 - 150
Control: 6 vials containing 25 mL of growth medium and algal inoculum (0.8 * 104 cells/mL)
Replicates: 3 vials per concentration, containing 25 mL of test solution and algal inoculum (0.8 * 104 cells/mL)
Negative controls: 1 vial per concentration, containing 25 mL of test solution without algal inoculum and 1 vial containing 25 mL of growth medium without algal inoculum (Blank) - Test organisms (species):
- other: Raphidocelis subcapitata (formerly called Pseudokirchneriella subcapitata), strain CCAP 278/4, in exponential growth phase
- Details on test organisms:
- Raphidocelis subcapitata (formerly called Pseudokirchneriella subcapitata), strain CCAP 278/4, in exponential growth phase
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- Temperature, 22 ± 2°C
- pH:
- 7.7-7.8
- Dissolved oxygen:
- 8.8-8.9 mg/L
- Conductivity:
- 675-676 µS/cm
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass vials containing 25 mL of test solution
- Aeration: None within the test period
- Renewal rate of test solution (frequency/flow rate): None
- Initial cells density: 0.8 x 10^4/ mL
- Control end cells density: 59.96 x 10^4/ mL
- Negative controls: 1 vial per concentration, containing 25 mL of test solution without algal inoculum and 1 vial containing 25 mL of growth medium without algal inoculum (Blank)
- No. of vessels per concentration (replicates): 3 vials per concentration, containing 25 mL of test solution and algal inoculum (0.8 * 10^4cells/mL)
- No. of vessels per control (replicates): 6 vials containing 25 mL of growth medium and algal inoculum (0.8 * 10^4 cells/mL)
GROWTH MEDIUM
- Standard medium used: yes OECD growth medium
OTHER TEST CONDITIONS
Temperature, 22 ± 2°C; continuous illumination (5190 - 5490 Lux), constant agitation (120 rpm).
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Daily: algae cell counting with the particle counter
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Test concentrations: 2.90 - 6.40 - 14.1 - 31.0 - 68.2 - 150 mg/L (nominal) - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 150 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- A measurement of the organic carbon was performed on the stock solution and two test concentrations to verify the stability of the sample (based on the organic carbon content present in the test solutions).
To follow-up the organic carbon in the test solutions, additional vessels, without algae, were prepared and placed under the test conditions.
The results obtained during this test are presented in Table below. - Validity criteria fulfilled:
- yes
- Conclusions:
- No significant inhibition of growth on Raphidocelis subcapitata was observed during this test at the
nominal concentration of 150 mg/L in the registered substance (maximum concentration tested). ErC50 is higher than 150 mg/L (nominal concentation).
Reference
Table: Measurement of organic carbon (mg O.C./L) in test solutions
Nominal test concentration (mg/L) | Theoretical O.C. (mg/L) | Solubilized O.C (mg O.C./L)/ % Day 0 | Solubilized O.C (mg O.C./L)/ % Day 1 | Solubilized O.C (mg O.C./L)/ % Day 2 | Solubilized O.C (mg O.C./L)/ % Day 3 | O.C. decrease at the end of the test (%) |
31.0 | 18.0 | 14.2/ 78.6 | 13.6/ 75.5 | 13.7/ 76.2 | 13.6/ 75.5 | 4.2 |
150 | 87.2 | 75.4/ 86.5 | 72.0/ 82.7 | 71.7/ 82.3 | 68.2/ 78.3 | 9.5 |
200 | 116.2 | 99.0/ 85.2 | 91.8/ 79.0 | 92.3/ 79.4 | 85.2 73.4 | 13.9 |
The amount of solubilized organic carbon measured in the test solutions is between 78.6 and 86.5% and the O.C. remain relatively stable over 72 hours under test conditions (greater than 73%).
Table: Growth rate and inhibition of algae growth after 72 hours
Nominal test concentration (mg/L) | Growth rate (days-1) | Growth rate inhibition (%) |
Control | 1.438 | / |
2.90 | 1.422 | 1.1 |
6.40 | 1.435 | 0.2 |
14.1 | 1.447 | - 0.6 |
31.0 | 1.443 | - 0.3 |
68.2 | 1.439 | - 0.1 |
150.0 | 1.406 | 2.2 |
No significant inhibition of growth on Raphidocelis subcapitata was observed during this test at the
nominal concentration of 150 mg/L in TOX20022 (maximum concentration tested).
Description of key information
No significant inhibition of growth on Raphidocelis subcapitata was observed during this test at the nominal concentration of 150 mg/L in the registered substance (maximum concentration tested). ErC50 is higher than 150 mg/L (nominal concentation).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 150 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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