2-chloro-5-methoxybenzene-1-sulfonamide

Toxicity to soil microorganisms

Administrative data

Endpoint:

toxicity to soil microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 217 (Soil Microorganisms: Carbon Transformation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

1

Reference substance name:

2-chloro-5-methoxybenzene-1-sulfonamide

Cas Number:

502187-53-3

Molecular formula:

C7H8ClNO3S

IUPAC Name:

2-chloro-5-methoxybenzene-1-sulfonamide

Specific details on test material used for the study:

Test substance: IN-F4106-009
Lot number: CCS-1213/STG-01/00112 BL
Purity: 97.2%

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

yes

Remarks:

Quartz sand

Test organisms

Test organisms (inoculum):

soil

Study design

Total exposure duration:

44 d

Remarks:

The test substance was incubated in a loamy sand soil over a period of 28 days (carbon
transformation) and 44 days (nitrogen transformation) at concentrations of 15.83 mg and
158.3 mg test item/kg soil dry weight.

Test conditions

Test temperature:

20 to 22°C

Moisture:

Nitrogen transformation test:
47% to 50% of WHCmax
Carbon transformation test:
44% to 47% of WHCmax

Organic carbon content (% dry weight):

1.22

Nominal and measured concentrations:

15.83 mg and 158.3 mg test item/kg soil dry weight.

Reference substance (positive control):

yes

Remarks:

Sodium chloride

Results and discussion

Effect concentrations

Key result

Duration:

28 d

Basis for effect:

other: There is no long-term impact of test substance on soil microbial nitrification and respiration processes up to and including concentrations of 158.3 mg test item/kg soil dry weight.

Results with reference substance (positive control):

Based on the results of this study, the reference item sodium chloride has a significant impact on nitrogen turnover and respiration activities of soil microflora when applied at 16 g/kg soil dry weight.
The reference item sodium chloride has long term influence on soil microflora and confirms the sensitivity of the test system.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

There is no long-term impact of test substance on soil microbial nitrification and respiration
processes up to and including concentrations of 158.3 mg test item/kg soil dry weight.

Executive summary:

The effects of test substance on soil microflora were assessed in a test that measured nitrogen transformation and short-term respiration following an application of IN-F4106 to soil. The test was conducted in accordance with the OECD-Guideline for the Testing of Chemicals, Soil Microorganisms: Nitrogen Transformation Test, Guideline 216 and OECD-Guideline for the Testing of Chemicals, Soil Microorganisms: Carbon Transformation Test, Guideline 217, dated 21 January 2000.

Test substance was incubated in a loamy sand soil over a period of 28 days (carbon transformation) and 44 days (nitrogen transformation) at concentrations of 15.83 mg and 158.3 mg test item/kg soil dry weight.

Deviations in nitrate levels and nitrate formation rates in soil treated with up to and including 158.3 mg test item/kg soil dry weight were low at day 44 and all rates were < 25% indicating no effect occurred in nitrogen transformation. Deviations of short-term respiration in soil treated with up to and including 158.3 mg test item/kg soil dry weight were low at day 28 and all rates were < 25% indicating no effect occurred in carbon transformation.

Test substance has no effect on nitrogen transformation or respiration at concentrations up to and including 158.3 mg test item/kg soil dry weight (deviations between treatments and controls for both nitrogen and carbon transformation tests were in the range of 25% deviation as specified by the OECD guidelines at day 44 and 28, respectively). The results indicate that there is a large margin of safety for test substance in microbial processes in soil.