Registration Dossier

Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08 Mar 2001 - 05 Jun 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: This study was conducted according to Good Laboratory Practice (GLP) and the method applied follows that described in the OECD Guideline for Testing of Chemicals 305 (Flow through fish test).

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report Date:
2001

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Lot RO-4562
Radiolabelling:
no

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
according to guidelne

Study design

Route of exposure:
aqueous
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
96 h

Test conditions

Hardness:
100 mg/L
Test temperature:
25
pH:
7.7-7.9
TOC:
0.9
Details on test conditions:
according to guidelne
Nominal and measured concentrations:
0.02 mg/L
0.002 mg/L
Details on estimation of bioconcentration:
HPLC

Results and discussion

Bioaccumulation factoropen allclose all
Type:
BCF
Value:
180
Basis:
normalised lipid fraction
Remarks on result:
other: Conc.in environment / dose:0.005 mg/L
Type:
BCF
Value:
145
Basis:
normalised lipid fraction
Remarks on result:
other: Conc.in environment / dose:0.05 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The mean BCF for CC-5 -V as determined in a flow through test system ammounts to 184 in this test system.
Executive summary:

Purpose

The purpose of this study was to determine the biocencentration potential of the test material CC-5 -V using Cyprinus carpio in a flow through assay to provide a rational basis for hazard estimation for the test item in aquatic environments.

Study Design

The bioconcentration factor of the test substance CC-5 -V to Cyprinus carpio was determined in a 96 -hour flow through test according to OECD 305.

Results

During the test no mortality was observed. Given test concentrations of CC-5 -V in the aquatic media of 20 µg/L and 2 µg/L yield BCFs of 78 and 290. From these data, a mean BCF of 184 can be calculated.

Conclusion

The mean BCF for CC-5 -V as determined in a flow through test system ammounts to 184 in this test system.