Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12 Dec 2000 - 05 Feb 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study was performed in compliance with the Good Laboratory Practice (GLP) regulations (revised in 1997, ENV/MC/CHEM(98)17). The method followed that described in the OECD Guidelines for Testing of Chemicals (Adopted: 4 April 1984) No 301C.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report Date:
2001

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Chemical Name: CC-5-V
Batch:
Appearance: (e.g. white powder)
Strorage: dark, at room temperature, tightly closed
Purity (HPLC): 99,98 %

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
>= 28 d
Initial test substance concentration
Initial conc.:
300 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
according to guideline
Reference substance
Reference substance:
aniline

Results and discussion

Test performance:
The reference substance showed 77 % degradation after day 21 of the experiment and later.
% Degradation
Parameter:
% degradation (test mat. analysis)
Value:
2
Sampling time:
28 d
Details on results:
The test substance showed 2 % degradation at day 28 of the experiment.

BOD5 / COD results

BOD5 / COD
Parameter:
COD
Value:
100.5 mg O2/g test mat.
Results with reference substance:
The reference substance showed 77 % degradation after day 21 of the experiment and later.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The test item CC-5-V was found to be NOT readily biodegradable under the described conditions.
Executive summary:

Purpose

The purpose of this assay was to identify the biodegradation potential of CC-5 -V in aquatic environments to provide a rational basis for environmental fata estimation for the test item.

Study Design

The test item CC-5 -V was investigated for its ready biodegradability in a MITI test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the micro organisms during exposure. As a reference item Aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.

Result

Biodegradation of CC-5 -V

After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28-day exposure period degradation rates of 2 % and 3 % were found.

Biodegradation of Aniline

The reference item Aniline was sufficiently degraded to 77 % after 21 days and thereafter, thus confirming the suitability of the used activated sludge inoculum.

Conclusion

CC-5 -V was found to be not ready biodegradable under the test conditions within 28 days.