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EC number: 223-564-1 | CAS number: 3962-66-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Oct 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not relevant
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: SampIes for the concentration analysis by TOC were taken at 0 and 48 hours after start of exposure.
The calculated results derived from TOC analysis gave mean concentrations 18.7 mg/L (saturated solution), 8.0 mg/L (1:2 solution), 5.4 mg/L (1:4 solution) and 1.7 mg/L (1:8 solution). - Vehicle:
- no
- Details on test solutions:
- For the preparation of the test solutions, a suspension with a nominal loading of 100 mg/L was ultrasonified (30 min) and stirred for approximately 24 h. This suspension was filtered through a glassfibre filter. The resulting solution served as the highest concentration (saturated solution). It was further diluted 1:2, 1:4, 1:8.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Source: Schering AG, exp. Toxicology, Berlin, Germany
Age: 0-24 h
Holding: parent animals were kept in glass vessels in water of the same quality and temperature as the experimental conditions. The animals were fed continuously with a laboratory culture of microalgae, e.g. Chorella vulgaris. Normal tap water was used as holding water, not polluted with harmful quantities of chlorine and organochlorine compounds, based on quartely measurements. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 264 mg/L CaCO3
- Test temperature:
- 21.3 - 21.6 °C. This is within the accepted range.
- pH:
- The pH varied between 7.1 and 7.2 at the beginning of the test and between 8.0 and 8.4 at the end of the test. There was a variation of more than one unit of pH from the beginning of the test to the end in all concentrations. However, this does not need to be considered especially as this is most likely not substance-related and is nor assumed to have an effect of the daphnids.
- Dissolved oxygen:
- 8.5 - 8.9 mg O2/L. This was within the accepted range.
- Conductivity:
- 727 µS/cm
- Nominal and measured concentrations:
- A saturated solution of a nominal 100 mg/l was used, this was then diluted 1:2, 1:4 and 1:8. The highest measured concentration was 18.7 mg/L, then 8.0, 5.4 and 1.7 mg/L.
- Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 20
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Photoperiod: 12 h ligh/12 h darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mobility was measured after 24 and 48 h. - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 18.7 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The EC50 (48 h) was slightly higher than the highest observed concentration (18.7 mg/L), which corresponds to the maximum solubility under the conditions of this test. This agreed well with the results of the limit test, where 50% of the daphnids were immobilized in the saturated solution.
- Results with reference substance (positive control):
- No positive control was used.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The study resulted in an EC50 (48 h) of > 18.7 mg/L (measured, saturated solution)
- Executive summary:
The purpose of the study was to determine the acute toxicity (immobilization, EC50/48 hours) of Delta-5 -Norandrostendion with Daphnia magna in order to evaluate the aquatic toxicity. The study was conducted according to OECD 202.
20 juvenile daphnids were used for each test solution and 20 for the tap water control. The daphnids were exposed for a period of 48 hours under static conditions. Immobilization was recorded at 24 hours and 48 hours. For the preparation of the test solutions a suspension with a nominal concentration of 100 mg/L was ultrasonified (30 min) and stirred for approximately 24 hours. This suspension was filtered through a glassfibre filter. The resulting solution served as the highest concentration (saturated solution). It was further diluted 1:2, 1:4, and 1:8.
Samples for the concentration analysis by TOC were taken at 0 and 48 hours after start of exposure. The calculated results derived from TOC analysis gave mean concentrations of 18.7 mg/L (saturated solution), 8.0 mg/L (1:2 solution), 5.4 mg/L (1:4 dilution), and 1.7 mg/L (1:8 dilution).
40% of the daphnids were immobilized at the highest test concentration after 48 h. Thus, an EC50 (48 h) of >18.7 mg/L (measured) and >100 mg/L (nominal) resulted.
Reference
Table 1: Cumulative immobilization (%) of Daphnia magna (n = 20) after exposure
Measured concentration | 24 hours | 48 hours |
(mg/L) | ||
0 | 0 | 0 |
Control (tap water) | ||
1.7 | 0 | 0 |
5.4 | 0 | 0 |
8 | 0 | 0 |
18.7 (saturated solution) | 5 | 40 |
Description of key information
The study resulted in an EC50 (48 h) > 18.7 mg/L (measured, saturated solution).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 18.7 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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