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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2005-3-11 - 2005-12-21
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
other: Body responsible for the test
Title:
Unnamed
Year:
2005
Report Date:
2005

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: C.8 Toxicity for earthworms : artificial soil test OECD 207: OECD Guideline for testing of Chemicals "Earthwor, Acute Toxicity Test"
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Batch No: 26/2004

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
Acetone

Test organisms

Test organisms (species):
other: Eisenia foetida
Details on test organisms:
TEST ORGANISM
- Common name:
- Source: Aqua-tera Svatopluk Ondrej Pozděchov, Czech Republik
- Age at test initiation (mean and range, SD): 2-3 months
- Weight at test initiation (mean and range, SD): 308 573 mg


ACCLIMATION
- Acclimation period: 24 h
- Acclimation conditions (same as test or not): same
- Health during acclimation (any mortality observed): no mortality

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
14 d

Test conditions

Test temperature:
20+/-2°C
Details on test conditions:
Soil characteristics (e.g. pH, % organic matter):
Basic substrate compound:

- 10% peat [ pH 5.5-6.0, moisture content 45-63% ]

- 20% china clay which consist from more then 50% kaolin

- 69-70% industrial silicic sand

- 0-1% calcium carbonate [powdery, chemically pure for pH
adjustment to 6.0+/-0.5]

All basic substrate compounds are mixed thoroughly and in
this homogenized mixture dried matter was analysed at
temperature 105deg. C = 87.1 % w/w

[ analysed by Sartorius MA 30 moisture analyzer ]


Artificial soil substrate compound:

For toxicity test on earthworm the defined artificial soil
was used as a supporting medium with demanded moisture

25-42%, consist of basic substrate and deionized water to
obtain adequately moist soil

-reference matter : chloroacetamide [ NH2-CO-CH2-Cl ]p.a.

-auxiliary matter: acetone p.a.
Nominal and measured concentrations:
preliminary test 0.1 - 1 - 10 - 100 - 1000 mg/kg dried matter
basic test: 640 - 800 - 1000 - 1280 - 1600 - 2000 mg/kg dried matter
Reference substance (positive control):
yes
Remarks:
chloroacetamide

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
1 463 other: mg/kg
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
LC0
Effect conc.:
800 other: mg/kg
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
LC100
Effect conc.:
> 2 000 other: mg/kg
Basis for effect:
mortality
Results with reference substance (positive control):
LC50= 18.4 mg/kg dried matter

Applicant's summary and conclusion