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EC number: 249-528-5 | CAS number: 29232-93-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1 Jun 1999 to 27Jul 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- 1987
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1100 (Acute Oral Toxicity)
- Version / remarks:
- 1998
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Pirimiphos-methyl
- EC Number:
- 249-528-5
- EC Name:
- Pirimiphos-methyl
- Cas Number:
- 29232-93-7
- Molecular formula:
- C11H20N3O3PS
- IUPAC Name:
- O-2-(diethylamino)-6-methylpyrimidin-4-yl O,O-dimethyl phosphorothioate
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Alpk:APfSD
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 8-12 weeks old
- Weight at study initiation: Males: 270-381 g, females: 191-241 g
- Fasting period before study: The rats were fasted overnight immediately prior to dosing.
- Housing: A maximum of 5 rats was housed per cage, sexes separately, in cages suitable for animals of this strain and the weight range expected during the course of the study.
- Diet and water: Supplied by an automatic system were available ad libitum.
- Acclimation period: The animals were housed under the experimental conditions for at least 5 days, prior to the start of the study.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 3
- Humidity (%): 30 - 70
- Air changes (per hr): A minimum of 15 changes per hour.
- Photoperiod (hrs dark / hrs light): 12 / 12
IN-LIFE DATES: 1 Jun 1999 to 27Jul 1999
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- maize oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 10 mg/kg - Doses:
- 500, 1000 and 2000 mg/kg
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations clinical signs: The animals were observed for signs of systemic toxicity twice following dosing on day 1. Subsequent observations were made daily, up to day 15.
- Frequiency of weighing: Prior to fasting (day -1), immediately before dosing (day 1) and on days 8 and 15.
- Clinical signs including body weight and systemic toxicity
- Post mortem: Examination of all thoracic and abdominal viscera. All abnormalities were recorded but tissues were not submitted for histopathological examination. - Statistics:
- LC50 values and 95% confidence intervals were determined.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 1 414 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- > 1 000 - < 2 000
- Mortality:
- Following a dose of 500 mg/kg and 1000 mg/kg, none of the animals died. Following a dose of 2000 mg/kg, all the animals were killed in extremis on days 2 or 3. Overview of the results were provided in table 1 in “Any other information on results incl. tables”
- Clinical signs:
- other: Signs of slight or moderate toxicity were seen in all animals, with complete recovery by day 5 in the males and day 8 in the females (some females had stained fur until day 10/13)
- Gross pathology:
- Following a dose of 500 mg/kg, one female had pelvic dilatation of the kidney. This is a common spontaneous finding which is considered to be unrelated to treatment. Following a dose of 1000 mg/kg, there were no macroscopic abnormalities in any animal. Following a dose of 2000 mg/kg, treatment-related abnormalities comprised staining/discharge from the eye, red nares, fluid stomach contents and (in one female) reddening of the pancreas.
Any other information on results incl. tables
Table 1. Cumulative mortality data
Dose level (mg/kg) |
Day Number |
Number of Deaths |
|
Male |
Female |
||
500 |
- |
0 |
0 |
15 (total) |
0/5 |
0/5 |
|
1000 |
- |
0 |
0 |
15 (total) |
0/5 |
0/5 |
|
2000 |
2 3 |
5 0 |
2 3 |
15 (total) |
5/5 |
5/5 |
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The acute oral LD50 of the test substance is estimated to be 1414 mg/kg (95% confidence limits 1000, 2000) to male and female rats.
- Executive summary:
Groups of five male and five female Alpk:APrSD rats received a single oral dose of 500, 1000 or 2000 mg/kg of the test substance in a study performed under GLP according to OECD TG 401. The animals were assessed daily for the following 14 days for any signs of systemic toxicity and their body weights were recorded at intervals throughout the study. Animals in extremis and those surviving to the end of the study were killed and subjected to a macroscopic examination post-mortem.
Following a dose of 500 mg/kg, none of the animals died. Signs of slight systemic toxicity were seen in most animals, with complete recovery by day 5. All animals showed an overall body weight gain during the study. There were no treatment-related abnormalities at examination post mortem. Following a dose of 1000 mg/kg, none of the animals died. Signs of slight or moderate toxicity were seen in all animals, with complete recovery by day 5 in the males and day 8 in the females. All animals showed an overall body weight gain during the study. There were no macroscopic abnormalities at examination post-mortem. Following a dose of 2000 mg/kg, all the animals were killed in extremis on days 2 or 3. At examination post mortem, treatment-related abnormalities comprised staining/discharge from the eye, red nares, fluid stomach contents and (in one female) reddening of the pancreas.
Based on these findings, the acute oral median lethal dose (LD50) of the test substance is estimated to be 1414 mg/kg (95% confidence limits 1000, 2000) to male and female rats.
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