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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to Read Across Statement attached in Section 13
- Reason / purpose for cross-reference:
- read-across source
- Preliminary study:
- The test guideline recommends a test concentration of the test substance of 10 - 40 mg/L Dissolved Organic Carbon (DOC). A test concentration of 20 mg/L DOC was used. The selected test concentration of 20 mg DOC/L corresponds to approximately 62 mg/L Acetic acid, oxo-, sodium salt.
It is anticipated that the selected test concentration causes no toxic effects to the microorganisms. The selected test concentration was tested in an additional inhibition control test assay and no toxic effects to the microorganisms were observed. - Test performance:
- No unusual observations during test or any other information affecting results were reported.
- Parameter:
- % degradation (DOC removal)
- Value:
- 10 - 20
- Sampling time:
- 28 d
- Remarks on result:
- other: mean value of two test assays
- Details on results:
- The test substance is considered as not readily biodegradable according to the OECD criteria.
- Results with reference substance:
- The reference substance (aniline) attained 91 % degradation after 14 days, based on DOC.
- Validity criteria fulfilled:
- yes
- Remarks:
- All validity criteria are fulfilled according to the applied OECD Guideline.
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test material attained 10 - 20 % degradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline 301A. The study was conducted under certificated GLP compliance and a well documented study report is available. The same result is expected for the target substance because the core chemical structures of EDDHA-moiety-containing constituents are the same in the source and in the target substance. Thus the same biodegradation rates are expected.
- Executive summary:
The DOC Die-Away Test is a batch test method to determine the ultimate aerobic biodegradability of a test substance in water. Mixtures of the test substance, a defined inorganic medium and a non pre-adapted inoculum (e.g. activated sludge or effluent from a municipal wastewater treatment plant) are incubated and aerated at a temperature of 22 +/- 2 °C for up to 28 days (Schwarz, 2010). Samples are taken at regular intervals to determine the Dissolved Organic Carbon concentration (DOC). The mean value of two test assays was used to determine the final biodegradation rate after the regulary exposure time. Thereby the test substance attained 10 - 20 % degradation. Based on the co-incubation with the reference substance anilin, it could be demonstrated that the test concentration used (20 mg/L DOC) was not toxic (inhibitory effect) on the sewage treatment bacteria flora. Anilin attained 91 % degradation after 14 days, indicating that the test system was suitable. In this experiment, the test substance was poorly biodegradable and is therefore considered as "not readily biodegradable" according to OECD criteria.
Reference
ACCOUNT OF TEST RESULTS
Test substance: Acetic acid.oxo-.sodium salt
Stock Solution, initial weight: 1233.7 mg/L
Dissolved Organic Carbon (DOC): 403 mg/L
DOC conc. at the test (nominal): 20 mg/L
Reference substance: aniline
Stock Solution, initial weight: 400.5 mg/L
Theoretical Organic Carbon (ThOC): 310 mg/L (calculated by initial weight)
DOC conc. at the test (nominal): 20 mg/L
Sample |
BC1 |
BC2 |
RS |
IH |
PC |
AC |
TS1 |
TS2 |
|
Test substance concentration [mg/L] | |
- |
- |
- |
61.7 |
61.7 |
61.7 |
61.7 |
61.7 |
|
Inoculum conc. [mg/L dry matter] |
30 |
30 |
30 |
30 |
- |
30 |
30 |
30 |
|
Demineralized water [mL/vessel] |
982 |
982 |
917 |
867 |
936 |
931 |
932 |
932 |
|
Mineral |
solution A |
10 |
10 |
10 |
10 |
10 |
10 |
10 |
10 |
medium |
solution B |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
[mL/L] |
solution C |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
solution D |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
|
Stock Solution; test substance [mL] |
- |
- |
- |
50 |
50 |
50 |
50 |
50 |
|
Stock Solution; reference subst.[mL] |
- |
- |
65 |
65 |
- |
- |
- |
- |
|
Mercury chloride sol.| [mL/vessel] |
- |
- |
- |
- |
1 |
1 |
- |
- |
|
pH values before; |
7.8 |
7.8 |
7.8 |
7.7 |
7.7 |
7.7 |
7.7 |
7.8 |
|
7.4 |
7.4 |
7.4 |
7.4 |
7.4 |
7.4 |
7.4 |
7.4 |
||
Inoculum (6 g/L) [mL/vessel] |
5 |
5 |
5 |
5 |
- |
5 |
5 |
5 |
|
Total test volume [mL] |
1000 |
1000 |
1000 |
1000 |
1000 |
1000 |
1000 |
1000 |
Legend: BC = blank control; RS = reference substance; IH = inhibition control; PC = abiotic control; AC = adsorption control; TS = test substance
Removal of Dissolved Organic Carbon (DOC) [%]:
Test duration [days] |
RS |
IH |
PC |
AC |
TS1 |
TS2 |
TS mv |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
-1 |
0 |
-5 |
3 |
-1 |
1 |
0 |
3 |
10 |
12 |
-3 |
7 |
7 |
5 |
6 |
7 |
96 |
53 |
1 |
7 |
9 |
10 |
10 |
10 |
92 |
52 |
-2 |
|
8 |
5 |
7 |
14 |
91 |
52 |
3 |
|
7 |
5 |
6 |
17 |
95 |
55 |
-3 |
|
12 |
12 |
12 |
21 |
93 |
54 |
1 |
|
8 |
6 |
7 |
24 |
95 |
57 |
6 |
|
13 |
13 |
13 |
27 |
98 |
59 |
3 |
|
17 |
16 |
17 |
28 |
97 |
57 |
4 |
|
11 |
10 |
11 |
Legend: BC = blank control; RS = reference substance; IH = inhibition control; PC = abiotic control; TS = test substance, AC = adsorption control; mv = mean value
Description of key information
Read across: OECD 301A, not readily biodegradable - 10 - 20 % degradation after 28 days
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
- Type of water:
- freshwater
Additional information
No experimental investigations concerning the biodegradability of the registered substance are available. Therefore, read-across from the structurally related substance Fe(Na)EDDHA (CAS 84539-55-9) was performed (BASF, 2010; Study No. 21G0753/093217). The DOC Die-Away Test is a batch test method to determine the ultimate aerobic biodegradability of the source substance Fe(Na)EDDHA in water. Mixtures of the test substance, a defined inorganic medium and a non pre-adapted inoculum (e.g. activated sludge or effluent from a municipal wastewater treatment plant) are incubated and aerated at a temperature of 22 +/- 2 °C for up to 28 days (Schwarz, 2010). Samples are taken at regular intervals to determine the Dissolved Organic Carbon concentration (DOC). The mean value of two test assays was used to determine the final biodegradation rate after the regular exposure time. Thereby the test substance attained 10 - 20 % degradation. Based on the co-incubation with the reference substance aniline, it could be demonstrated that the test concentration used (20 mg/L DOC) was not toxic (inhibitory effect) on the sewage treatment bacteria flora. Aniline attained 91 % degradation after 14 days, indicating that the test system was suitable. In this experiment, the test substance was poorly biodegradable and is therefore considered as "not readily biodegradable" according to OECD criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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