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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
3 h EL50 >1000 mg/L, NOEL 460mg/L; OECD 209, EU Method C.11 and ISO Standard 8192
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 460 mg/L
Additional information
In the key study, the potential for the test material to cause toxicity to activated sludge was investigated in a study conducted in accordance with the standardised guidelines OECD 209, EU Method C.11 and ISO Standard 8192 under GLP conditions. The study was awarded a reliability score of 1 in accordance with the principles for assessing data quality set forth by Klimisch et al. (1997).
The influence of the test material on the respiration rate of activated sludge of a predominantly domestic sewage was investigated after a contact time of 3 hours.
The test material was not sufficiently soluble to allow the preparation of a 10 g/L stock solution in water. Therefore, test material and Milli-RO water mixtures were magnetically stirred for a period of 24.5 to 25.5 hours. Subsequently, synthetic medium, sludge and Milli-RO water were added resulting in the required loading rates. Optimal contact between the test material and test medium was ensured by applying continuous aeration and stirring during the 3-hour exposure period. Thereafter, oxygen consumption was recorded for approximately 10 minutes.
The final test was performed based on the result of a preceding combined limit/range-finding test. Five loading rates were tested at nominal concentrations of 46, 100, 220, 460 and 1000 mg/L. Five replicates per loading rate and six replicates for an untreated control group were tested.
No statistically significant inhibition of the respiration rate of the sludge was recorded at or below a loading rate of 460 mg per litre. At 1000 mg/L the inhibitory effect of the test material on aerobic waste water (activated sludge) bacteria was 30 %.
The EL10, EL20 and EL50 loading rates were 340, 810 and >1000 mg/L, respectively.
The batch of activated sludge was tested for sensitivity with the reference material 3,5-dichlorophenol and showed normal sensitivity. The study met the acceptability criteria and was considered valid.
Under the conditions of the study, the test material was not toxic to waste water bacteria (domestic activated sludge) at or below a loading rate of 460 mg/L (NOEL).
Supporting information is available in the form of a disregarded study that was conducted to investigate the inhibition of the growth of a pure culture of Pseudomonas fluorescens. The test material was present in mineral oil and administered as a water-leachate described as a dense emulsion. Accurate assessment of growth by optical density was not possible and analysis comprised dissolved organic carbon measurements, which are not selective for the test material. On this basis, the study was awarded a reliability score of 3 in accordance with the criteria set forth by Klimisch et al. (1997).
In the study, plate counts conducted after 5 hours showed that the water-leachate of the test material reduced cell numbers by 16 % compared to control inocula.
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