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EC number: 954-793-1
CAS number: -
The objective of this study was to evaluate the influence of the test item on the immobilization of Daphnia magna.
For this purpose, juvenile daphnids were exposed to a nominal test item concentration of 100 mg/L (limit test) over 48 hours, in an open static system. The daphnids were observed for immobilization 24 and 48 hours after placing in the aqueous test item solution. The study comprised of one test item group with four test vessels containing five daphnids each, i.e. 20 daphnids in total. Additionally, one control group (20 daphnids) was used.
The test item concentration in the reconstituted water was not quantified at the start and the end of this study. Because of the low water solubility (1.7e-12 mg/L), the compound cannot be detected with standard analytical methods and the development of an analytical method with a sufficiently low detection and quantification limit is complex.
Due to the absence of any adverse effects at the saturation concentration, the study was performed without analytical concentration verification.
After exposure of daphnids to a nominal concentration of 100 mg/L for 48 hours, the following results were obtained:
Daphnids exposed to an aqueous preparation of a nominal concentration of 100 mg/L of the test item were not affected.
The following EC50 values for daphnids were determined:
24h EC50: >1.7e-12 mg/L (nominal >100 mg/L)
48h EC50: >1.7e-12 mg/L (nominal >100 mg/L)
Under the conditions of the present study, an aqueous solution of nominal 100 mg/L of the test item revealed no aquatic toxicity in the test system.
The 48h EC50 was >1.7e-12 mg/L (nominal >100 mg/L) and, thus, could not be determined in this study.
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