[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Remarks:

this test was not carried out initially to meet the REACH registration requirement, hence the fact that it is not carried out under GLP.

Test material

Test material information

Constituent 1

Reference substance name:

Dioctadecyl ether

Cas Number:

6297-03-6

Molecular formula:

C36H74O

IUPAC Name:

Dioctadecyl ether

Constituent 2

Reference substance name:

Dieicosyl ether

Cas Number:

502696-65-3

Molecular formula:

C40H82O

IUPAC Name:

Dieicosyl ether

Constituent 3

Reference substance name:

Didocosyl ether

Cas Number:

77456-42-9

Molecular formula:

C44H90O

IUPAC Name:

Didocosyl ether

Constituent 4

Reference substance name:

Methanesulphonic acid

EC Number:

200-898-6

EC Name:

Methanesulphonic acid

Cas Number:

75-75-2

Molecular formula:

CH4O3S

IUPAC Name:

methanesulphonic acid

Constituent 5

Reference substance name:

Octadecan-1-ol

EC Number:

204-017-6

EC Name:

Octadecan-1-ol

Cas Number:

112-92-5

Molecular formula:

C18H38O

IUPAC Name:

octadecan-1-ol

Constituent 6

Reference substance name:

Icosan-1-ol

EC Number:

211-119-4

EC Name:

Icosan-1-ol

Cas Number:

629-96-9

Molecular formula:

C20H42O

IUPAC Name:

icosan-1-ol

Constituent 7

Reference substance name:

Docosan-1-ol

EC Number:

211-546-6

EC Name:

Docosan-1-ol

Cas Number:

661-19-8

Molecular formula:

C22H46O

IUPAC Name:

docosan-1-ol

Constituent 8

Reference substance name:

betaine glycine mesylate

Molecular formula:

C6NO5

IUPAC Name:

betaine glycine mesylate

Constituent 9

Reference substance name:

Octadecyl betainate mesylate

Molecular formula:

C24NO4

IUPAC Name:

Octadecyl betainate mesylate

Constituent 10

Reference substance name:

Eicosyl betainate mesylate

Molecular formula:

C26NO4

IUPAC Name:

Eicosyl betainate mesylate

Constituent 11

Reference substance name:

Docosyl betainate mesylate

Cas Number:

Docosyl betainate mesylate

Molecular formula:

C28NO4

IUPAC Name:

Docosyl betainate mesylate

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

SFE: Solubilized fraction or forming a homogeneous suspension in water, obtained from a loading rate in mg / L of the product to be studied.
To prepare each ESF, a quantity of product to be studied is brought into contact with test medium at the load rate tested, in a closed container, with orbital agitation for 24 hours at approximately 110 rpm, at 20 ° +/- 2 ° C.
After shaking, the vials containing the loading levels are subjected to ultrasound for 1 minute. The aqueous fraction of each loading rate (FSE) is then recovered.
The SFE are used immediately for the performance of the test but the results are expressed as loading rate.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

225 mg/L +/- 50 mg (CaCO3)

pH:

7.8 +/- 0.5

Dissolved oxygen:

< 7 mg/L

Salinity:

Ca/Mg = 4/1 = +/-0.5

Nominal and measured concentrations:

Nominal: 90mg/L, 80mg/L, 62mg/L, 48mg/L, 37mg/L

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 83.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

ca. 87 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Results with reference substance (positive control):

Positive control: K2Cr2O7
EC50-48h = 0.92mg/L (to be valid, the value should be between 0.60 mg/L and 2.1mg/L)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The toxicity of the test item to aquatic invertebrate was performed according to the OECD 202 guideline.

The results were the following:

EC50 48h=83.8mg/L

EC50 24h=87mg/L