Registration Dossier

Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
17 February 2004
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of relevant results.
Cross-reference
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report Date:
2004

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
(Date of inspection: 02 December 2002 Date of signature: 13 February 2003)
Type of method:
HPLC estimation method
Media:
other: methanol

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): TIPX
- Substance type: Colourless liquid.
- Physical state: Liquid.
- Lot/batch No.: 042028.
- Storage condition of test material: Approximately 4ºC in the dark.
Radiolabelling:
no

Study design

Test temperature:
(Column temperature for HPLC analysis) 25°C.

HPLC method

Details on study design: HPLC method:
EQUIPMENT
- Apparatus: HPLC, Agilent Technologies 1100, incorporating autosampler and workstation.
- Column: Phenosphere NEXT CN, 5µ (250 x 4.6 mm id).
- Flow rate: 1 ml/min
- Injection volume: 10 µl

MOBILE PHASES
- Type: methanol: water (55:45 v/v)
- pH: 5.9

DETERMINATION OF DEAD TIME
The dead time was determined by measuring the retention time of formamide (purity, quoted by supplier, ≥99.5%, 160 mg/l solution in methanol).
- Dead time and reference standards: 210 nm
- Sample: 210 nm

REFERENCE SUBSTANCES
- Identity: acetanilide, phenol, atrazine, isoproturon, triadimenol, linuron, naphthalene, endosulfan-diol, fenthion, phenanthrene, diclofop-methyl and DDT. See concentrations of each reference substance in Table 1.

EVALUATION
The capacity factor was calculated using the following formula and the log10Koc value determined with reference to the calibration curve;
k = (tr - t0) / t0
where:
k = capacity factor
tr = retention time (min)
t0 = dead time (min)


Results and discussion

Adsorption coefficient
Type:
log Koc
Value:
4.22
Temp.:
25 °C
Remarks on result:
other: Temperature as column temperature.

Results: HPLC method

Details on results (HPLC method):
See results of absorption coefficient of the sample in Table 2.

- Retention times of reference substances used for calibration: See Table 3.

- Details of fitted regression line (log k' vs. log Koc): See the Figure, as attached.

- Average retention data for test substance: 12.176 mins.

Any other information on results incl. tables

Table 1 Reference substances

Standard

Purity (%),

quoted by supplier

Concentration (mg/l)

Acetanilide

~99

128

Phenol

99.99

166

Atrazine

99.2

133

Isoproturon

99

116

Triadimenol

98.5

127

Linuron

99

112

Naphthalene

³99.0

116

Endosulfan-diol

99.9

116

Fenthion

96

153

Phenanthrene

³97

105

Diclofop-methyl

99.3

111

DDT

98

147

Table 2 Adsorption Coefficient of the Sample

Injection

Retention Time (mins)

Capacity Factor (k)

log10k

log10Koc

1

12.178

2.49

0.396

4.22

2

12.174

2.49

0.396

4.22

Table 3 Calibration Results

Standard

Retention Time (mins)

Mean Retention Time (mins)

Capacity Factor (k)

Log10k

Log10Koc

Injection 1

Injection 2

Formamide

3.49

3.493

3.492

-

-

-

Acetanilide

3.834

3.836

3.835

9.84 x 10-2

-1.01

1.25

Phenol

3.76

3.76

3.76

7.69 x 10-2

-1.11

1.32

Atrazine

4.247

4.245

4.246

0.216

-0.665

1.81

Isoproturon

4.558

4.556

4.557

0.305

-0.515

1.86

Triadimenol

5.185

5.186

5.186

0.485

-0.314

2.4

Linuron

5.324

5.326

5.325

0.525

-0.28

2.59

Naphthalene

5.809

5.805

5.807

0.663

-0.178

2.75

Endosulfan-diol

6.15

6.142

6.146

0.76

-0.119

3.02

Fenthion

7.971

7.972

7.972

1.28

0.108

3.31

Phenanthrene

8.684

8.694

8.689

1.49

0.173

4.09

Diclofop-methyl

12.492

12.487

12.49

2.58

0.411

4.2

DDT

35.359

35.337

35.348

9.12

0.96

5.63

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The adsorption coefficient (Koc) of the test material has been determined to be 1.68E+04, log10 Koc 4.22.
Executive summary:

Adsorption Coefficient has been determined to be 1.68 x 104, log10Koc 4.22, using the HPLC screening method, Method C19 of Commission Directive 2001/59/EC.