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Ecotoxicological information

Toxicity to soil microorganisms

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Link to relevant study record(s)

Description of key information

According the OECD 216 & 217 and GLP studies, the chronic toxicity of the test matrial (EC 953-378-2) on terrestrial micro-organisms is considered to be :

- OECD 217 : NOEC - 28d = 320 mg a.i/kg dry soil.

- OECD 216 : NOEC - 28d > 1000 mg a.i/kg dry soil.

Key value for chemical safety assessment

Long-term EC10 or NOEC for soil microorganisms:
320 mg/kg soil dw

Additional information

Toxicity on micro-organisms (OECD 216 & 217, GLP):

This study was performed to assess the long-term effect of the test material after a single exposure, on the nitrogen transformation and carbon transformation activities of soil micro-organisms.


Soil microorganisms were exposed to the test item, at five treatment concentrations (10, 32, 100, 320 and 1000 mg/kg dry weight soil) for 28 days at temperatures of between 18 to 22 °C in the dark. The test soil was a sandy loam soil obtained from a site in Rugby, Warwickshire, England that had received no pesticides or fertilisers for 5 years prior to sampling.


To summarize, the lack of treatment related effects on nitrogen transformation activity in soil at each of the treatment levels prevented calculation of EC values and demonstrated the NOEC to be 1000 mg/kg dry weight soil. Whilst the NOEC level was considered to be 320 mg/kg dry weight soil in carbon transformation, it was considered that test item showed no long-term inhibitory effect on carbon transformation in soil at the concentrations tested in this study.