Reaction mass of 2- (palmitoylamino)ethyl acrylate and 2-(stearoylamino)ethyl acrylate

Hydrolysis

Link to relevant study record(s)

Reference

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 111 (Hydrolysis as a Function of pH)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source (i.e. manufacturer or supplier) and lot/batch number of test material: provided by sponsor, Lot number 91112Y
- Purity.: 92.45%


STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test sample was stored in a dark storage place at room temperature.
- Stability and homogeneity of the test material in the vehicle/solvent under test conditions (e.g. in the exposure medium) and during storage: stability


TREATMENT OF TEST MATERIAL PRIOR TO TESTING
0.2 mg/L

Radiolabelling:

no

Analytical monitoring:

yes

Details on sampling:

- Sampling intervals for the parent/transformation products:
Take out 2.5mL from test solution to pH measurement at initiation ; After 5 days， two test solutions in each pH were taken out from the dry thermo bath and were cooled to room temperature， and then were pretreated and analyzed; Take out 1mL(transfer pipette) Fill up to10mL (tetrahydrofuran， volumetric flask) for LC-MS/MS analysis

Buffers:

- pH:
- Type buffer: pH 4, pH 7, pH 9

Duration:

5 d

pH:

9

Temp.:

50 °C

Initial conc. measured:

0.181 mg/L

Remarks:

peak2; The test item is a multi-constituent substance, so two peaks of LC-MS/MS was hydrolysis analysis respectively.

Duration:

5 d

pH:

7

Temp.:

50 °C

Initial conc. measured:

0.146 mg/L

Remarks:

peak2; The test item is a multi-constituent substance, so two peaks of LC-MS/MS was hydrolysis analysis respectively.

Duration:

5 d

pH:

4

Temp.:

50 °C

Initial conc. measured:

0.191 mg/L

Remarks:

peak 2; The test item is a multi-constituent substance, so two peaks of LC-MS/MS was hydrolysis analysis respectively.

Duration:

5 d

pH:

9

Temp.:

50 °C

Initial conc. measured:

0.183 mg/L

Remarks:

peak 1; The test item is a multi-constituent substance, so two peaks of LC-MS/MS was hydrolysis analysis respectively.

Duration:

5 d

pH:

7

Temp.:

50 °C

Initial conc. measured:

0.151 mg/L

Remarks:

peak1; The test item is a multi-constituent substance, so two peaks of LC-MS/MS was hydrolysis analysis respectively.

Duration:

5 d

pH:

4

Temp.:

50 °C

Initial conc. measured:

0.189 mg/L

Remarks:

peak1; The test item is a multi-constituent substance, so two peaks of LC-MS/MS was hydrolysis analysis respectively.

Number of replicates:

1

Preliminary study:

Tier 1, the test item was observed the hydrolyisis percent for 5 days under pH4, pH7 and pH9 at 50℃. This substance is a multi-constituents substance, has two peaks when analysis.The residual percentage of peak 1 is 47.4%, 38.2%, 10.9% under pH4, pH7 and pH 9 respectively; of peak 2 is 35.5%, 50.4%, 0.181% under pH4, pH7 and pH 9 respectively.
The test item is not hydrolytically stable at pH4, pH7 and pH9, under 50℃.

Transformation products:

not specified

Remarks:

Any hydrolysis products could not be detected at all pH.The reason is regarded as detection limit of the test item was low(0.2mg/L)and the amounts of hydrolysis products were very small.

Details on hydrolysis and appearance of transformation product(s):

not specified
Any hydrolysis products could not be detected at all pH.The reason is regarded as detection limit of the test item was low(0.2mg/L)and the amounts of hydrolysis products were very small.

% Recovery:

39.1

pH:

9

Temp.:

50 °C

Duration:

5 d

Remarks on result:

other: peak2; The test item is a multi-constituent substance, so two peaks of LC-MS/MS was hydrolysis analysis respectively.

% Recovery:

50.4

pH:

7

Temp.:

50 °C

Duration:

5 d

Remarks on result:

other: peak2; The test item is a multi-constituent substance, so two peaks of LC-MS/MS was hydrolysis analysis respectively.

% Recovery:

35.5

pH:

4

Temp.:

50 °C

Duration:

5 d

Remarks on result:

other: peak2; The test item is a multi-constituent substance, so two peaks of LC-MS/MS was hydrolysis analysis respectively.

% Recovery:

10.9

pH:

9

Temp.:

50 °C

Duration:

5 d

Remarks on result:

other: peak1; The test item is a multi-constituent substance, so two peaks of LC-MS/MS was hydrolysis analysis respectively.

% Recovery:

38.2

pH:

7

Temp.:

50 °C

Duration:

5 d

Remarks on result:

other: peak1; The test item is a multi-constituent substance, so two peaks of LC-MS/MS was hydrolysis analysis respectively.

% Recovery:

47.4

pH:

4

Temp.:

50 °C

Duration:

5 d

Remarks on result:

other: peak1; The test item is a multi-constituent substance, so two peaks of LC-MS/MS was hydrolysis analysis respectively.

Remarks on result:

not measured/tested

Remarks:

Tier 2 test was not performed because the measurement of hydrolysis rate for test item(multi- components substance) was difficult.

Conclusions:

The test item is not hydrolytically stable at pH4, pH7 and pH9.

Executive summary:

In this study(OECD 111, GLP)， the preliminary test(Tier 1) in OECD guideline No. 111 was performed to evaluate the hydrolysis of test item. The test item is not hydrolytically stable at pH4, pH7 and pH9 after 5 days exposure, test item has residual percentage of is 10.9%-50.4% at pH4 , pH7 and pH 9 in the 5th day.Therefore, T1/2 of the test item is ≤5 days.

Tier 2 test was not performed because the measurement of hydrolysis rate for test item(multi- components substance) was difficult. Tier 3 test was also performed to identify hydrolysis products. However, any hydrolysis products could not be detected at all pH,since the detection limit of the test item was low(0.2mg/L)and the amounts of hydrolysis products were too low to detect.

It is concluded the test item is not hydrolytically stable at pH4, pH7 and pH9 after 5 days exposure.

Description of key information

In the hydrolysis study（OECD 111, GLP, reliability 2) , test item has residual percentage of is 10.9%-50.4% at pH4 , pH7 and pH 9 in the 5th day.Therefore, T1/2 of the test item is ≤5 days.

 

 

Key value for chemical safety assessment

Half-life for hydrolysis:

5 d

at the temperature of:

50 °C