Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 457-310-8 | CAS number: -
The genotoxic potential of tetraammineplatinum diacetate was analysed in a bacterial reverse mutation (Ames) assay, conducted according to OECD Test Guideline 471 and to GLP. A dose range finding test was performed using Salmonella typhimurium strain TA100 and Escherichia coli strain WP2uvrA. Triplicate cell cultures were exposed to tetraammineplatinum diacetate at up to 5000 µg/plate, both in the presence and absence of metabolic activation by rat liver fraction S9 (alongside appropriate vehicle and positive controls). These cultures were then inspected for signs of cytotoxicity and for the presence of revertant colonies. Since no cytotoxicity was observed, in the main experiment triplicate cultures of S. typhimurium strains TA98, TA1535 and TA1537 were exposed to the test material at concentrations of 100, 330, 1000, 3330 and 5000 µg/plate, both in the presence and absence of metabolic activation by S9 (alongside appropriate vehicle and positive controls), and were incubated for 48 hours at 37 °C before being inspected for signs of cytotoxicity and for the presence of revertant colonies.
Significant cytotoxicity was not observed for any of the tested strains, at any of the tested concentrations. Significant, dose-related increases in the number of observed revertant colonies were seen for S. typhimurium strains TA98 (up to 2.3 -fold in the presence of S9) and TA1537 (up to 13 -fold and 18 -fold in the absence and presence of S9 respectively), and E. coli strain WP2uvrA (up to 3.2 -fold and 6 -fold in the absence and presence of S9 respectively). No significant, dose-related increases were seen in S. typhimurium strain TA98 in the absence of S9, or in TA100 and TA1535 (both in the absence and presence of S9). Under the conditions of this assay, tetraammineplatinum diacetate showed mutagenic potential.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again