1-Propene, 1,3,3,3-tetrafluoro-

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

effects on growth of green algae

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Sampling method: 100 µL taken and added to appropriate vials to maintain conconcentation
- Sample storage conditions before analysis: Stored at 2 - 10˚C and shipped at the end of the test, analyzed at the laboratory within 24 hours

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
The test material was purged through test media for different periods of time at a rate of 20 mL/min. The gas flow was assessed using a soap-bubble flow meter. To incease the availablity of CO2 for the alga when inclubated in a closed vessel, extra NaHCO3 was added resultin in a concentration of 100mg/L.

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Strain: NIVA CHL-1
- Source: In house laboratory culture

ACCLIMATION
- Culturing media and conditions: Same as test
- Any deformed or abnormal cells observed: No

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

pH:

Time 0: pH 8.3 - 8.6
72 hours: pH 9.4 - 10.2

Nominal and measured concentrations:

Purging media at 20 mL/min for 2.5 hours resulted in a mean measured concentration of 170 mg/L at 72 hours.

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL
- Type : Closed (All glass and airtight closed with a screw cap holding a septum)
- Aeration: No
- Initial cells density: 10000 cells/mL
- Control end cells density: 58000 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 1

GROWTH MEDIUM
- Standard medium used: Yes

OTHER TEST CONDITIONS
- Sterile test conditions: Yes
- Light intensity and quality: 71 - 82 uE.m(-2)s(-1)

EFFECT PARAMETERS MEASURED
- Determination of cell concentrations: Counting chamber and spectrophotometer

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

170 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 170 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

> 170 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

< 170 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

biomass

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

< 170 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

biomass

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 170 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

biomass

Details on results:

- Exponential growth in the control: Yes
- Observation of abnormalities: No
- Any observations that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium

Results with reference substance (positive control):

- Results with reference substance valid - Yes
- EC50: 72 hr cell growth inhibition at 0.78 mg/L, 72 hr growth rate reduction at 1.6 mg/L

Validity criteria fulfilled:

yes

Remarks:

Although the pH range from time 0 to 72 hours varied by more than 1.5 units, this was due to the study being conducted in a closed system. This change in pH did not effect the exponential algal growth and was not considered to adversely affected the study

Conclusions:

In an OECD TG 201 and EU method C3 followed toxicity study on green algae, the ErC50 and NOErC values of the test substance were determined to be > 170 and 170 mg/L, respectively, based on mean measured concentrations.

Description of key information

In an OECD TG 201 and EU method C3 followed toxicity study on green algae, the ErC50 and NOErC values of the test substance were determined to be > 170 and 170 mg/L, respectively, based on mean measured concentrations.

Key value for chemical safety assessment

EC10 or NOEC for freshwater algae:

170 mg/L