Registration Dossier
Registration Dossier
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EC number: 224-305-5 | CAS number: 4297-95-4
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April/May 2019
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Sodium phenylphosphinate
- EC Number:
- 224-305-5
- EC Name:
- Sodium phenylphosphinate
- Cas Number:
- 4297-95-4
- Molecular formula:
- C6H7O2P.Na
- IUPAC Name:
- sodium phenylphosphinate
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Activated sludge from a biologic sewage treatment plant was used as inoculum. The chosen plant treats mostly domestic sewage.
The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, 67435 NW-Lachen-Speyerdorf.
The sludge was filtrated, washed with test medium (2x) and re-suspended in test medium. It was then aerated until use. The dry matter was determined to contain 4.8 g of suspend-ed solids/L. - Duration of test (contact time):
- ca. 28 d
Initial test substance concentration
- Initial conc.:
- ca. 20 mg/L
- Based on:
- TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- The medium was prepared from the stock solutions. The stock solution of the positive control was prepared and its DOC was measured. The stock solution of the test item was prepared and its DOC was measured. The inoculum was taken from its source, washed, aerated and the dry matter was determined.
The test vessels were filled with medium and inoculum. Then, all flasks were aerated for 72 hours with purified, CO2-free, moistened air to purge the system of CO2.
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 0.3
- Sampling time:
- 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
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