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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin corrosion: in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report date:
2013

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 430 (In Vitro Skin Corrosion: Transcutaneous Electrical Resistance Test Method (TER))
Deviations:
no
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
1-(3,5-dichloro-4-fluorophenyl)-2,2,2-trifluoroethan-1-one
EC Number:
829-719-5
Cas Number:
1190865-44-1
Molecular formula:
C8H2Cl2F4O
IUPAC Name:
1-(3,5-dichloro-4-fluorophenyl)-2,2,2-trifluoroethan-1-one
Test material form:
liquid

In vitro test system

Test system:
isolated skin discs
Source species:
rat
Cell type:
non-transformed keratinocytes
Cell source:
other: pelt of humanely killed young Wistar strain rat
Source strain:
Wistar
Details on animal used as source of test system:
Not available
Vehicle:
unchanged (no vehicle)
Control samples:
yes, concurrent negative control
yes, concurrent positive control
Amount/concentration applied:
Not reported
Duration of treatment / exposure:
24 hours
Number of replicates:
Three

Results and discussion

In vitro

Results
Irritation / corrosion parameter:
transcutaneous electrical resistance (in kΩ)
Run / experiment:
Mean
Value:
22.7
Vehicle controls validity:
not applicable
Negative controls validity:
valid
Remarks:
13.4
Positive controls validity:
valid
Remarks:
0.97

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
Based on the results of the in vitro TER study, the substance is unlikely to be corrosive to skin in vivo.
Executive summary:

An in vitro skin corrosion study following the principles of OECD TG 430 (transcutaneous electrical resistance assay) was conducted with the substance under non-GLP conditions. Due to the poor level of documentation, the reliability of the test cannot be assessed. Skin discs were prepared from the pelt of a humanely killed young Wistar strain rat. Three discs were used to test the corrosion potential of the test substance, a negative and a positive control. All substances were applied as such to the surfaces of the skin discs for a period of 24 hours, and then removed with a jet of warm water. The electrical resistance of the skin was then measured. The mean electrical resistance measured was 22.7 kOhms for the test substance, 13.4 kOhms for the negative control and 0.97 kOhms for the positive control. Based on these results, the substance is considered unlikely to be corrosive to the skin in vivo.

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