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EC number: 829-719-5
CAS number: 1190865-44-1
No immobilised animals were observed at the test concentration of 10 mg/L (and lower test concentrations), whereas all of the test organisms were found to be immobile at the three highest test concentrations after 24 hours of exposure.
The acute toxicity of the test substance to freshwater invertebrates (Daphnia magna) was determined under GLP in a 48-hour static test according to the OECD TG 202 (2004). Twenty daphnids, aged <24 hours, were randomly divided into groups of five and exposed to each test concentration in four replicates. The nominal concentrations were 4.6, 10, 22, 46 and 100 mg/L. A control (test water without test item) was tested in parallel. The analytically determined concentrations of the substance in the test media at the start and the end of the exposure ranged between 93 and 105% of the nominal values and between 79 and 91% of nominal at the end of the test. Although in the lowest concentration the test item was observed to be under 80% of the nominal concentration at the end of the exposure (79%), this is a minor deviation and this concentration is below all effect values. The effect levels were therefore calculated on the basis of nominal test concentrations. No immobilisation of test organisms was observed at test concentrations of 4.6 and 10 mg/L after 24 or 48 hours, whereas all test organisms were immobilised at the three higher test concentrations at these time points. The 48-hour EC50 value based on immobilisation was determined to be 15 mg/L, with 95% confidence limits of 10 and 22 mg/L.
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