Reaction mass of 1,3-diisopropylbenzene and 1,4-diisopropylbenzene

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

1976

Materials and methods

Test guideline

Qualifier:

no guideline followed

Guideline:

other: Study from 1976 (no guidelines available at the time the study was performed).

Principles of method if other than guideline:

One or two New Zealand Albino rabbits (m or f) in each dosing group were dosed with undiluted TS over 24 hrs. Observations were made for toxic signs and the viscera of the test animals were examined macroscopically.

GLP compliance:

no

Remarks:

Study from 1976 (GLP was not compulsory at the time the study was performed).

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of 1,3-diisopropylbenzene and 1,4-diisopropylbenzene

EC Number:

905-459-9

Molecular formula:

C12H18

IUPAC Name:

Reaction mass of 1,3-diisopropylbenzene and 1,4-diisopropylbenzene

Details on test material:

Lot/batch No.: XF 958

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

Initial body weights: 2100 - 2200 g (m) and 1800 - 2200 g (f)

Administration / exposure

Type of coverage:

not specified

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

No further data

Duration of exposure:

24 hrs

Doses:

1000, 2000, 3160, 5010 or 7940 mg/kg bw

No. of animals per sex per dose:

1000 mg/kg bw: 1 m
2000 mg/kg bw: 1 f
3160 mg/kg bw: 1 m
5010 mg/kg bw: 1 f
7940 mg/kg bw: 1 m / 1 f

Control animals:

not specified

Details on study design:

Survivors were sacrificed 14 days after dosing.

Statistics:

No data

Results and discussion

Preliminary study:

Slighty irritating according to Draize socre: not irritating.

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 3 160 mg/kg bw

Mortality:

1000 mg/kg bw: 0/1 m
2000 mg/kg bw: 0/1 f
3160 mg/kg bw: 0/1 m
5010 mg/kg bw: 1/1 f (time to mortality: 4 days)
7940 mg/kg bw: 0/1 m and 1/1 f (time to mortality: 2 days)

Survival time: 2 - 4 days

Clinical signs:

other: reduced appetite and activity (4 - 7 days in survivors), increasing weakness, collapse and death.

Gross pathology:

Decedents: Haemorrhagic areas of the lungs, liver hyperaemia, enlarged gall bladder and GI tract inflammation.
Survivors: viscera appeared normal.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The dermal LD50 value for male and female rabbits was > 3160 mg/kg bw.

Executive summary:

In this older study one or two New Zealand Albino rabbits (m or f) in each dosing group were dosed with undiluted TS at doses of 1000, 2000, 3160, 5010 or 7940 mg/kg bw over 24 hrs. Observations were made for toxic signs and the viscera of the test animals were examined macroscopically. The dermal LD50 value for male and female rabbits was given with > 3160 mg/kg bw. Toxic signs included reduced appetite and activity, increasing weakness, collapse and death. In decedents, haemorrhagic areas of the lungs, liver hyperaemia, enlarged gall bladder and GI tract inflammation were noted, while in survivors viscera appeared normal.

This is the lowest value, as the other study give a higher one (13700 mg/kg)