2,3-bis((2-mercaptoethyl)thio)-1-propanethiol

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25-Oct-1989 ¿ 21-May-1990

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

4-mercaptomethyl-3,6-dithia-1,8-octanedithiol

IUPAC Name:

4-mercaptomethyl-3,6-dithia-1,8-octanedithiol

Test material form:

aerosol dispenser: not specified

Remarks:

migrated information: aerosol

Details on test material:

- Lot/batch No.: 111

Test animals

Species:

rat

Strain:

Crj: CD(SD)

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

whole body

Vehicle:

air

Analytical verification of test atmosphere concentrations:

yes

Remarks:

gravimetric determination of DMPT

Duration of exposure:

4 h

Concentrations:

The test animals received an average gravimetric exposure concentration of 4.8 mg/l of DMPT with a nominal exposure concentration of 71 mg/l. A total of 340 grams of test substance was used during the exposure. This was considered a maximum attainable exposure level based on pre-study trials. A distribution sample taken during the second hour of exposure demonstrated less than 10% variability compared to the routine sampling point.

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

All animals were held for a 21-day post-exposure observation period and received daily detailed physical observations pretest through Day 22, and body weight measurements on days 1, 2, 3, 5, 8, 15 and 22. After the post-exposure period, all animals were sacrificed and a complete gross postmortem examination was conducted.

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC0

Effect level:

4.8 mg/L air (analytical)

Based on:

test mat.

Effect levels 2

Sex:

male/female

Dose descriptor:

LC0

Effect level:

71 mg/L air (nominal)

Based on:

test mat.

Mortality:

All animals survived the duration of the study.

Clinical signs:

other: Observations noted during exposure were limited to decreased activity and eye closure. Signs exhibited by animals upon removal from the chamber and during the two-hour post-exposure observation period on Day 1 included labored breathing, lacrimation, nasa

Body weight:

Substantial weight losses were observed during the first 2 to 4 days following exposure. Recovery of weight occurred over time and half of the
animals were in excess of their pre-exposure body weight after 2 weeks. Most of the females continued to gain weight during Week 3, however,
Test Week 3 body weights for the males were less than Test Week 2 because of a water system malfunction.

Gross pathology:

Red/brown discoloration of the skin was observed in 5/5 males and 3/5 females. Red/tan discoloration of the lungs and turbinates were observed in 2/5 male animals. The toxicologic significance of the above findings in a single dose group in the absence of a control group and based entirely on gross findings is equivocal. Other postmortem findings which were observed grossly occurred sporadically and were not considered to be related to the test article.

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Remarks:

Migrated information

Conclusions:

The inhalation toxicity (aerosol study, MMAD 4.0 µm for 4 h of DMPT in Sprague-Dawley rats is considered to be harmful (LC50 ¿ 5.0 mg/l/4h). No mortalities were observed in this study.